产品名称
抗-人IgM(μ-链特异性)(F(ab′)2片段−过氧化物酶 山羊抗, affinity isolated antibody, buffered aqueous solution
biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Application
山羊多克隆抗人IgM(μ链特异性)F(ab′)2片段过氧化物酶抗体可用于通过显色、化学发光或荧光免疫化学或免疫组织化学技术检测和定量人血清和生物体液中的IgM水平。 使用特异性HRP偶联的山羊抗人IgMμ链作为二抗,对从EBV+人B细胞建立的EBV+BCL进行ELISA分析。二抗在室温下孵育2小时。
过氧化物偶联的山羊F(abμ)2片段抗人IgM(′-链特异性)已被用作二抗,以使用ELISA检测针对汉坦病毒抗原的人IgM。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
免疫球蛋白M(IgM)抗体出现在感染过程的早期。 IgM抗体负责错配输血中的红细胞凝集。IgM的水平可能随疾病或感染的状况而变化。
辣根过氧化物酶(HRP)是一种可催化显色底物转化的酶,如邻苯二胺(OPD)、4-氯-1-萘酚3,3'、5,5'-四甲基联苯胺(TMB)、3,3′-二氨基联苯胺(DAB)或 2,2′-连氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS);化学发光底物如CPS-3(增强的腔)和荧光底物如Ampliflu™ Red分别成为可检测的发色团、发光体或荧光剂。
辣根过氧化物酶(HRP)是一种可催化显色底物转化的酶,如邻苯二胺(OPD)、4-氯-1-萘酚3,3'、5,5'-四甲基联苯胺(TMB)、3,3′-二氨基联苯胺(DAB)或 2,2′-连氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS);化学发光底物如CPS-3(增强的腔)和荧光底物如Ampliflu™ Red分别成为可检测的发色团、发光体或荧光剂。
当针对纯化的人IgA、IgG、IgM、Bence Jones Kappa和Bence Jones Lambda骨髓瘤蛋白进行测试时,山羊多克隆抗人IgM(μ链特异性)F(ab′)2片段过氧化物酶抗体对人IgM具有特异性。
Immunogen
纯化的人IgM
Physical form
0.01 M磷酸盐缓冲液(pH 7.4,含有带保存剂的1%牛血清白蛋白)溶液。
Preparation Note
按照Wilson, M.B.,和Nakane, P.K.在由阿姆斯特丹Elsevier/北荷兰生物医学出版社出版的免疫荧光及相关染色技术(1978)一书中所描述的高碘酸盐法而制备。
Legal Information
Ampliflu is a trademark of Sigma-Aldrich Co. LLC
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signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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