产品名称
抗-生物素−过氧化物酶 山羊抗, affinity isolated antibody, buffered aqueous solution
biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:2,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
使用 HRP 标记的山羊抗生物素抗体，通过蛋白质印迹分析 U937 和 BHK 细胞裂解物中的生物素标记蛋白。
可能取代亲和素以减少非特异性结合。
在一些应用中，生物素化探针与亲和素的定位会产生高背景水平。抗生物素试剂可替代亲和素，以减少非特异性结合。
山羊抗生物素过氧化物酶抗体可用于直接 ELISA 分析（稀释度为 1：2,000）。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
生物素是活生物体和培养物中细胞所需的必需维生素。生物素与蛋白亲和素或细菌来源的链霉亲和素的高结合亲和力已被用于设计用于检测抗原与抗体的相互作用的免疫测定法。山羊抗生物素过氧化物酶抗体可用于许多可能将生物素引入靶标的应用。
通过 Ouchterlony 双扩散和免疫电泳与生物素载体蛋白复合物测定特异性和反应性。未观察到与载体蛋白的反应性。
Immunogen
生物素-KLH 结合物
Physical form
0.01 M磷酸盐缓冲液，pH 7.4，含有1％牛血清白蛋白和防腐剂。
Preparation Note
通过 Wilson,M.B. 和 Nakane,P.K. 在《免疫荧光及相关染色技术》(Immunofluorescence and Related Staining Techniques) 中描述的过碘酸盐法制备（Elsevier/North Holland Biomedical Press 出版社，Amsterdam,p215 (1978)）。
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signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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