biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
IgA 抗体在粘膜免疫中发挥着重要作用,可与免疫原/病原体结合,限制其进入粘膜。 抗小鼠 IgA(α-链特异性)-过氧化物酶抗体可用于测定肠液中的分泌型 IgA (sIgA)。它也可用于直接ELISA。山羊抗小鼠 IgA(α-链特异性)-过氧化物酶抗体与小鼠 IgA 发生特异性反应。
Immunogen
纯化小鼠 IgA
Application
ELISA 采用 HRP 标记的山羊抗小鼠 IgA 作为二抗,1:10000 稀释,37 度孵育 1 小时,检测小鼠粪便提取物中的 IgA 水平。
抗小鼠 IgA(α-链特异性)-过氧化物酶抗体可用于改良间接 DIBA(斑点免疫结合试验)。
Physical form
0.01 M磷酸盐缓冲液,pH 7.4,含有1%牛血清白蛋白和防腐剂。
Preparation Note
采用 Wilson,M.B. 和 Nakane,P.K. 在 Immunofluorescence and Related Staining Techniques 中描述的过碘酸盐法制备,Elsevier/North Holland Biomedical Press,Amsterdam,P 215 (1978)。
Disclaimer
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
A C Rodrigues et al.
Journal of applied microbiology, 89(3), 404-414 (2000-10-06)
The effect of Saccharomyces boulardii on the immune system was evaluated, comparing germ-free Swiss/NIH mice monoassociated with the probiotic with germ-free mice. Saccharomyces boulardii colonized the gut of germ-free mice and survived the gastrointestinal conditions. An increase in sIgA production
Simona P Pfister et al.
Nature communications, 11(1), 1978-1978 (2020-04-26)
There is the notion that infection with a virulent intestinal pathogen induces generally stronger mucosal adaptive immunity than the exposure to an avirulent strain. Whether the associated mucosal inflammation is important or redundant for effective induction of immunity is, however
The Immune Response in Mice Immunized with Lactobacillus acidophilus LF221 - A Potential Probiotic Strain.
Irena Rogelj
Food Technology and Biotechnology, 37(3), 153-158 (1999)
Sidonia Fagarasan et al.
Nature reviews. Immunology, 3(1), 63-72 (2003-01-04)
Immunoglobulin A is the most abundant immunoglobulin isotype in mucosal secretions. In this review, we summarize recent advances in our understanding of the sites, mechanisms and functions of intestinal IgA synthesis in mice. On the basis of these recent findings
Hao Feng et al.
Frontiers in plant science, 8, 910-910 (2017-06-18)
Rotavirus is the leading cause of severe diarrheal disease among newborns. Plant-based rotavirus vaccines have been developed in recent years and have been proven to be effective in animal models. In the present study, we report a bivalent vaccine candidate
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