A5044

单克隆抗 α-辅肌动蛋白小鼠抗

Name: 单克隆抗 α-辅肌动蛋白 小鼠抗
Brand: SIGMA

clone BM-75.2, ascites fluid

别名:

Anti-BDPLT15

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关于此项目

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162107

Conjugate:

unconjugated

Clone:

BM-75.2, monoclonal

Application:

indirect immunofluorescence
microarray
western blot

Species reactivity:

mouse, bovine, human, chicken

Citations:

133

Technique(s):

indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable

Uniprot accession no.:

P12814

主要文件

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产品名称

单克隆抗 α-辅肌动蛋白小鼠抗, clone BM-75.2, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BM-75.2, monoclonal

mol wt

antigen 100 kDa

contains

15 mM sodium azide

species reactivity

mouse, bovine, human, chicken

technique(s)

indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable

isotype

IgM

UniProt accession no.

P12814

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... ACTN1(87)
mouse ... Actn1(109711)

Application

单克隆抗α-肌动蛋白抗体（稀释1：500) 可用于天然蛋白质结合测定。它还可用于蛋白质印迹、微阵列、免疫组化和免疫荧光显微镜。

可以用于骨骼肌组织研究和肌动蛋白膜锚定部位研究。

采用单克隆小鼠抗-α肌动蛋白（克隆 EA-53），以1:1000的稀释度对新生和成年小鼠心脏的心肌细胞进行免疫荧光分析。

Biochem/physiol Actions

α-肌动蛋白调节肌肉收缩和细胞骨架组织。它促进细胞迁移并用作肌动蛋白交联剂。在异质性肥厚性心肌病和青少年起病性房颤患者中，已发现α-辅肌动蛋白基因发生突变。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

α-辅肌动蛋白是肌肉以及非肌肉细胞中的一种100kD肌动蛋白结合蛋白。在平滑肌中，组织的密集体和斑块中存在α-辅肌动蛋白，而它与定义了正常骨骼肌中肌肉肉瘤的Z盘有关。单克隆抗-α-辅肌动蛋白抗体可用于免疫印迹，以研究α-辅肌动蛋白的膜锚定位点和免疫化学鉴定。它也可以用于免疫荧光研究α-辅肌动蛋白在培养的细胞和组织中的定位。鼠抗α辅肌动蛋白抗体与鸡成纤维细胞发生特异性反应。该产品还显示出对小鼠和人类细胞的反应性。

编码α-辅肌动蛋白的基因定位于人类染色体11q13.2。α-辅肌动蛋白属于血影蛋白（spectrin）超家族。

Immunogen

牛乳腺上皮细胞 (BMGE) 的细胞骨架部分。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

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存储类别

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Protein Interactions with the Glucose Transporter Binding Protein GLUT1CBP That Provide a Link between GLUT1 and the Cytoskeleton.

Robert C. Bunn, Mari Anne Jensen

Molecular and Cellular Biology, 10(4), 819-832 (1999)

The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis.

Thomas A Hawkins et al.

Development (Cambridge, England), 135(6), 1147-1156 (2008-02-08)

The mechanisms that regulate sarcomere assembly during myofibril formation are poorly understood. In this study, we characterise the zebrafish sloth(u45) mutant, in which the initial steps in sarcomere assembly take place, but thick filaments are absent and filamentous I-Z-I brushes

Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells.

C M Laukaitis et al.

The Journal of cell biology, 153(7), 1427-1440 (2001-06-27)

To investigate the mechanisms by which adhesions form and disperse in migrating cells, we expressed alpha 5 integrin, alpha-actinin, and paxillin as green fluorescent protein (GFP) fusions. All localized with their endogenous counterparts and did not perturb migration when expressed

Leiomodin and tropomodulin in smooth muscle.

C A Conley

American journal of physiology. Cell physiology, 280(6), C1645-C1656 (2001-05-15)

Evidence is accumulating to suggest that actin filament remodeling is critical for smooth muscle contraction, which implicates actin filament ends as important sites for regulation of contraction. Tropomodulin (Tmod) and smooth muscle leiomodin (SM-Lmod) have been found in many tissues

Autophagic-lysosomal inhibition compromises ubiquitin-proteasome system performance in a p62 dependent manner in cardiomyocytes.

Zongwen Tian et al.

PloS one, 9(6), e100715-e100715 (2014-06-25)

Intracellular protein degradation is primarily performed by the ubiquitin-proteasome system (UPS) and the autophagic-lysosomal pathway (ALP). The interplay between these two pathways has been rarely examined in intact animals and the mechanism underlying the interplay remains unclear. Hence, we sought

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单克隆抗 α-辅肌动蛋白 小鼠抗

clone BM-75.2, ascites fluid

选择尺寸

关于此项目

主要文件

属性

产品名称

biological source

conjugate

antibody form

antibody product type

clone

mol wt

contains

species reactivity

technique(s)

isotype

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

相关类别

说明

Application

Biochem/physiol Actions

Disclaimer

General description

Immunogen

Other Notes

安全信息

存储类别

wgk

flash_point_f

flash_point_c

文件

分析证书(COA)

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单克隆抗 α-辅肌动蛋白小鼠抗