产品名称
抗-小鼠 IgG（全分子）-碱性磷酸酶 山羊抗, affinity isolated antibody, buffered aqueous solution
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:7,000-1:21,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
使用碱性磷酸酶偶联的山羊抗小鼠IgG作为二抗，在5%BSA/TBS中以1:5000的稀释度在室温下进行1小时的核或线粒体蛋白提取物的蛋白质印迹分析。底物使用5-溴-4-氯-3-吲哚基磷酸酯/硝基蓝四唑（Sigma）。
在含有终浓度为0.5M NaCl的TBS/Tween中，使用碱性磷酸酶缀合的山羊抗小鼠IgG（μg/ ml）作为二抗，对支气管肺泡液中的表面活性蛋白A进行检测。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析（1篇论文）
蛋白质免疫印迹分析（1篇论文）
Biochem/physiol Actions
可与所有小鼠Igs结合。
小鼠IgG1、IgG2a、IgG2b和IgG
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
通过免疫特异性纯化从山羊抗小鼠IgG抗血清中获得亲和力分离的抗原特异性抗体，该过程可基本上去除所有山羊血清蛋白，包括免疫球蛋白（其不可与小鼠IgG特异性结合）。通过与0.2%戊二醛的蛋白交联，将山羊抗小鼠IgG与VII-S型Sigma碱性磷酸酶（产品编号P 5521）偶联。 偶联前，通过免疫电泳（IEP）对比小鼠IgG和正常小鼠血清，确定抗小鼠IgG（整个分子）对小鼠IgG具有免疫特异性。偶联前，通过Ouchterlony双扩散法（ODD）发现该抗体与小鼠IgG1、IgG2a、IgG2b和IgG反应。偶联前，通过免疫电泳（IEP）鉴定抗体及其纯度。抗体制品电泳后进行针对抗山羊IgG和抗山羊全血清的扩散，可形成单条沉淀弧。
Immunogen
纯化的小鼠 IgG
Physical form
在0.05 M Tris（pH 8.0）中的溶液，含1%牛血清白蛋白、1 mM MgCl2和15 mM叠氮化钠。
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存储类别
10 - Combustible liquids
wgk
WGK 2
法规信息
常规特殊物品
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