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A5441

Anti-β-Actin (ACTB) Antibody

Name: Anti-β-Actin (ACTB) Antibody
Brand: SIGMA

mouse monoclonal, AC-15

别名:

单克隆抗-β-Actin

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关于此项目

UNSPSC Code:

12352203

NACRES:

NA.41

Conjugate:

unconjugated

Clone:

AC-15, monoclonal

Application:

ELISA (i), IF, IHC (p), WB

Citations:

10789

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产品名称

单克隆抗 β-肌动蛋白抗体小鼠抗, clone AC-15, ascites fluid

biological source

mouse

Quality Level

300

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

AC-15, monoclonal

mol wt

antigen 42 kDa

contains

15 mM sodium azide

species reactivity

sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig

should not react with

Dictyostelium discoideum

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts, western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts

isotype

IgG1

UniProt accession no.

P60709

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

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General description

在鸡砂囊超薄组织冷冻切片的染色中，抗体对致密体和由结蛋白和膜相关的致密斑所占据的纵向通道连接的连续致密体进行了标记。它不会对成人的心脏和骨骼肌进行染色，除非是受到了非肌肉细胞样本的污染，或是使用了胚胎组织。

抗-β-肌动蛋白单克隆抗体（小鼠IgG1亚型）来自于免疫小鼠骨髓瘤细胞和脾细胞相互融合而产生的AC-15杂交瘤细胞。肌动蛋白是最保守的真核蛋白之一，在哺乳动物和鸟类中至少表达6种异构体。其中四种代表肌肉组织的分化标志物，而两种在几乎所有细胞中都有所发现。现有三种α-肌动蛋白（α-骨骼肌、α-心脏和 α-平滑肌），一种β-肌动蛋白（β-非肌肉）和两种γ-肌动蛋白（γ-平滑肌和γ-非肌肉）。肌动蛋白异构体显示>90%的整体序列同源性，但在其18个NH2-末端残基中只有5060%的同源性。肌动蛋白的NH2-末端区域似乎是一个主要的抗原区域，可能参与了肌动蛋白与肌球蛋白等其他蛋白的相互作用。该抗体可用于丙酮固定的冷冻切片、EM制品和显微注射实验的染色。B5、乙醇、methacam或Bouin′s溶液可用作固定剂。抗体所识别的表位对福尔马林固定和石蜡包埋具有抗性。

Immunogen

略微修改的β-细胞质肌动蛋白N末端肽，Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys，偶联至KLH。

Application

单克隆小鼠抗肌动蛋白抗体被用作一种上样对照，用于对来自大鼠背根神经节共培养物的免疫沉淀蛋白进行western blot分析。

单克隆小鼠抗肌动蛋白抗体被用作大鼠肝脏蛋白裂解液western blot分析的一种上样对照。

小鼠源抗-β-肌动蛋白单克隆抗体可用于：

免疫荧光染色
免疫印迹
免疫组织化学
用作蛋白芯片的对照品

Biochem/physiol Actions

抗β-肌动蛋白单克隆抗体能够识别位于肌动蛋白β-亚型N-端上的表位。在免疫印迹（42 kDa处的条带）、培养细胞的免疫荧光染色和免疫组织化学分析中，该抗体能特异性标记各种组织和物种中的β-肌动蛋白。

肌动蛋白和肌球蛋白是许多类型细胞的组成部分，参与运动、胞质分裂、细胞质流动、分泌和吞噬等诸多细胞过程。不同物种和组织来源细胞中的肌动蛋白的免疫学和生理特性非常相似。

Physical form

以含有15mM叠氮化钠作为防腐剂的腹水形式提供。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

想要查看肌动蛋白抗体选择指南，请访问 www.sigmaaldrich.com/actin。

Disclaimer

除非在我们的产品目录或产品随附的其他公司文档中另有说明，我们的产品仅供研究使用并不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途、任何类型的食用或应用于人或动物。

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cells.

Jodi L MacKeil et al.

Cellular signalling, 62, 109342-109342 (2019-06-09)

Recent reports show that protein kinase A (PKA), but not exchange protein activated by cAMP (EPAC), acts in a cell autonomous manner to constitutively reduce the angiogenic sprouting capacity of murine and human endothelial cells. Specificity in the cellular actions

The maximal cytoprotective function of the heat shock protein 27 is dependent on heat shock protein 70

Sreedharan R, et al.

Biochim. Biophys. Acta Gen. Subj., 1813(1), 129-135 (2011)

A STAT3-based gene signature stratifies glioma patients for targeted therapy.

Melanie Si Yan Tan et al.

Nature communications, 10(1), 3601-3601 (2019-08-11)

Intratumoral heterogeneity is a hallmark of glioblastoma (GBM) tumors, thought to negatively influence therapeutic outcome. Previous studies showed that mesenchymal tumors have a worse outcome than the proneural subtype. Here we focus on STAT3 as its activation precedes the proneural-mesenchymal

HIV brain latency as measured by CSF BcL11b relates to disrupted brain cellular energy in virally suppressed HIV infection.

Lucette A Cysique et al.

AIDS (London, England), 33(3), 433-441 (2018-11-27)

We investigated whether HIV brain latency was associated with brain injury in virally suppressed HIV infection. Observational cross-sectional and longitudinal study. The study included 26 virally suppressed HIV-infected men (61.5% with HIV-associated neurocognitive disorder) who undertook cerebrospinal fluid (CSF) analyses

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Cell & bioscience, 9, 64-64 (2019-08-17)

Chronic hepatitis B virus (HBV) infection is one of the most serious global public health problems. The role of steroid receptor coactivator 3 (SRC-3) in HBV biosynthesis is unknown. The aim of this study is to investigate the function of

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实验方案

Western Blot Protocol - Immunoblotting or Western Blot

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Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

Western Blotting实验方法 - 免疫印迹或Western Blot实验方法

带有用于不同印迹过程工作流程步骤的Western blot实验方法，描述了蛋白从SDS聚丙烯酰胺凝胶(SDS-PAGE)到硝酸纤维素膜(NC膜)的电泳转移。也称为免疫印迹实验方法。

全球贸易项目编号

货号	GTIN
A5441-.2ML	04061838443557
A5441-.5ML	04061838443564
A5441-100UL	04061838443571

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