mouse
unconjugated
ascites fluid
primary antibodies
AC-15, monoclonal
antigen 42 kDa
15 mM sodium azide
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
Dictyostelium discoideum
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts
western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts
IgG1
research pathology
dry ice
−20°C
unmodified
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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10 - Combustible liquids
WGK 3
Not applicable
Not applicable
输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。
为方便起见,与您过往购买产品相关的文件已保存在文档库中。
如何查找产品货号
在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).
示例
其它示例:
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
输入内容 1.000309185)
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如何查找COA批号
批号可以在产品标签上"批“ (Lot或Batch)字后面找到。
如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。
如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。
如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。
部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Beta Actin has a molecular weight of 42 kDa.
This antibody will detect mouse beta actin. When used for western blotting, the secondary antibody may react with mouse IgG from the sample resulting with a bands at 50 kDa and 25 kDa (reducing conditions) or approximately 150 kDa (non-reducing conditions). It is best to use a directly conjugated antibody for immunohistochemistry or immunofluorescent assays. We offer Product numbers A3854, peroxidase conjugated.
We have not determined the light chain used by monoclonal antibody AC-15. 95% of mouse antibodies have kappa light chains.
While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93:2234-2238, (1996).
This antibody is ascities fluid. The amount of protein present and the amount of IgG1 present is indicated on the Lot-specific Certificate of Analysis.
We recommend using Product No. A9044, rabbit anti-mouse IgG, peroxidase conjugate as a secondary antibody. We also offer this same antibody directly conjugated to peroxidase as Product No. A3854.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
Although actin is one of the most conserved eukaryotic proteins, it is expressed as six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of the six isoforms represent differentiation markers of muscle tissues. The other two are found in practically all cells.
Loading controls in western blotting application.
Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.
Leverage the inherent immune ability of an animal to generate antibodies that bind to specific molecules.
带有用于不同印迹过程工作流程步骤的Western blot实验方法,描述了蛋白从SDS聚丙烯酰胺凝胶(SDS-PAGE)到硝酸纤维素膜(NC膜)的电泳转移。也称为免疫印迹实验方法。
Western blot protocol with workflow steps for different blot procedures, describing the electrophoretic transfer of proteins from SDS polyacrylamide gels to sheets of nitrocellulose. Also known as Immunoblotting protcol.
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