A5691
抗-肌动蛋白，α-平滑肌-碱性磷酸酶抗体，小鼠单克隆
clone 1A4, purified from hybridoma cell culture
别名:
单克隆抗-肌动蛋白，α-平滑肌, 单克隆抗-肌动蛋白，α-平滑肌 - 碱性磷酸酶 小鼠抗, SMA
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选择尺寸
关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
1A4, monoclonal
Application:
ELISA, IHC (p), WB
Citations:
77
biological source
mouse
conjugate
alkaline phosphatase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
1A4, monoclonal
form
buffered aqueous glycerol solution
mol wt
antigen ~42 kDa
technique(s)
ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections, western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
isotype
IgG2a
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Acta2(11475)
rat ... Acta2(81633)
species reactivity
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
General description
其抗体（也称为抗α-Sm-1）对α-平滑肌肌动蛋白的单一异构体具有特异性。它在免疫印迹试验中可与α-平滑肌肌动蛋白发生特异性反应，并可在冷冻或福尔马林固定的、或石蜡包埋的组织切片中对平滑肌细胞进行标记。
肌动蛋白是一种高度保守的蛋白质，是所有细胞类型中细胞骨架和收缩结构的主要组成部分。肌动蛋白含量与分化类型以及细胞和组织的功能状态有关。肌动蛋白具有两种不同的聚集形式，即球状或纤维状，并且在脊椎动物中至少具有六种不同亚型。肌动蛋白表现出大于90％的序列同源性，但每种亚型都具有不同的NH 2-末端序列。同工型由三种α肌动蛋白（骨骼肌，心脏，平滑肌），一种β肌动蛋白（β-非肌肉）和两种γ肌动蛋白（γ平滑肌和γ非肌肉）组成。
Immunogen
N-末端合成的α-平滑肌肌动蛋白十肽。
Application
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
使用单克隆抗ACTA2抗体对来自牛主动脉的平滑肌细胞进行免疫细胞化学分析。首先将细胞在玻璃盖玻片上生长，并在4％的50％丙酮/乙醇中固定10分钟。
对固定在1%PFA中的角质细胞进行免疫细胞化学，并与小鼠单克隆抗平滑肌肌动蛋白（14A）以1:100的稀释度孵育。
小鼠单克隆抗肌动蛋白，α−平滑肌-碱性磷酸酶抗体可用于小鼠和人体组织的免疫组织化学应用。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫组织化学（1篇论文）
免疫组织化学（1篇论文）
Physical form
含有1 mM MgCl2、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠（用作防腐剂）的0.05M Tris缓冲液，pH 8.0中的溶液。
Other Notes
想要查看肌动蛋白抗体选择指南，请访问 www.sigmaaldrich.com/actin。
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存储类别
10 - Combustible liquids
wgk
WGK 2
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
此项目有
Immunohistochemical analysis of Sonic hedgehog signalling in normal human urinary tract development.
Dagan Jenkins et al.
Journal of anatomy, 211(5), 620-629 (2007-09-14)
Studies of mouse mutants have demonstrated that Sonic hedgehog (SHH) signalling has a functional role in morphogenesis and differentiation at multiple sites within the forming urinary tract, and urinary tract malformations have been reported in humans with mutations that disrupt
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Systemic sclerosis (SSc) is a connective tissue disorder characterized by fibrosis of the skin and inner organs, vasculopathy, and immunological abnormalities. Recent insights on the implication of STAT3, AKT, and Wnt/β-catenin in fibrosis have prompted us to investigate, in a
Izabela Rozenberg et al.
Arteriosclerosis, thrombosis, and vascular biology, 30(5), 923-930 (2010-03-06)
Enhanced endothelial permeability leading to intimal accumulation of low-density lipoproteins (LDL) stimulates the formation of atherosclerotic lesions. Histamine is known to increase vascular permeability. Whether this affects the formation of atherosclerotic lesions, however, remains elusive. Apolipoprotein E-null (ApoE(-/-)) mice treated
J V Jester et al.
Investigative ophthalmology & visual science, 40(9), 1959-1967 (1999-08-10)
Recent studies indicate that transforming growth factor (TGF)beta is a potent inducer of corneal myofibroblast differentiation and expression of smooth muscle-specific, alpha-actin (alpha-SMA). Although TGFbeta is known to enhance synthesis of extracellular matrix proteins and receptors, little is known about
Maryellen Sandor et al.
Eplasty, 17, e1-e1 (2017-01-26)
Objective: Benchtop methods were evaluated for preclinical inflammation/capsule formation correlation following implantation of human acellular dermal matrices. Methods: Dermal matrices were compared with native dermis for structure (histology, scanning electron microscopy), collagen solubility (hydroxyproline), enzymatic susceptibility (collagenase), and thermal stability
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