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Merck
CN

A6003

牛血清白蛋白 来源于牛血清

lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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关于此项目

化学文摘社编号:
9048-46-8
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.25
MDL number:
MFCD00130384
Biological source:
bovine
Assay:
≥96% (agarose gel electrophoresis), ≥96%
Form:
lyophilized powder
Technique(s):
microbiological culture: suitable
Impurities:
≤0.01% fatty acid (GC)

主要文件

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biological source

bovine

assay

≥96% (agarose gel electrophoresis), ≥96%

form

lyophilized powder

Quality Level

300

Gene Information

bovine ... ALB(280717)

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

free fatty acid content

≤0.01%

technique(s)

microbiological culture: suitable

impurities

≤0.01% fatty acid (GC)

pH

7

solubility

water: soluble (50 mg/ml)

UniProt accession no.

P02769

application(s)

hematology

foreign activity

BT virus, none detected, VSV virus, none detected

storage temp.

2-8°C

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相关类别

General description

BSA是已知的脂肪酸（FA）载体。因此，控制用于培养细胞系的特定FA′非常重要，因为不同细胞系对特定的脂肪酸可能有不同的敏感性。因此，不含脂肪酸的BSA是细胞培养实验的有用工具，培养实验中必须严格控制特定脂肪酸的含量，从而使研究人员能够使用对其细胞系有特异性的特定脂肪酸。不含脂肪酸的白蛋白还为细胞培养中特定脂肪酸的添加提供了最佳和最大结合位点。使用不含FA的BSA还可以缓解人们对非不含FA的BSA中可能含有内源FA′的担忧。
牛血清白蛋白（BSA）是一种含量丰富的血浆蛋白，有助于调节血液的胶体渗透压。BSA可用于多种不同应用，例如细胞培养、免疫化学和蛋白质定量。

该BSA产品经过专门测试，具有非常低的FA含量/无脂肪酸状态。

Biochem/physiol Actions

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

Features and Benefits

  • 冷乙醇分级
  • 基本上不含脂肪酸

Preparation Note

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
通常称为第五组分(Cohn fraction V)；本品采用改进的Cohn氏冷乙醇分级分离法制备。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000500772
0000500772
0000489870
0000489870
0000490326

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Kevin Huynh et al.
Metabolites, 8(2) (2018-04-25)
Macrophages are abundant within adipose tissue depots where they are exposed to fatty acids, leading to lipid accumulation. Herein, we have determined the effects of various fatty acids on the macrophage lipidome. Using targeted mass-spectrometry, we were able to detect
Wendy W S Yue et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(11), 5144-5153 (2019-02-24)
G protein-coupled receptor (GPCR) signaling is crucial for many physiological processes. A signature of such pathways is high amplification, a concept originating from retinal rod phototransduction, whereby one photoactivated rhodopsin molecule (Rho*) was long reported to activate several hundred transducins
Ting Wang et al.
Journal of translational medicine, 13, 152-152 (2015-05-09)
Osteoinduction and proliferation of bone-marrow stromal cells (BMSCs) in three-dimensional (3D) poly(ε-caprolactone) (PCL) scaffolds have not been studied throughly and are technically challenging. This study aimed to optimize nanocomposites of 3D PCL scaffolds to provide superior adhesion, proliferation and differentiation
Esmee Koedoot et al.
Nature communications, 10(1), 2983-2983 (2019-07-07)
Ttriple-negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype. Enhanced TNBC cell motility is a prerequisite of TNBC cell dissemination. Here, we apply an imaging-based RNAi phenotypic cell migration screen using two highly motile TNBC cell
G I Lancaster et al.
Nature communications, 7, 10626-10626 (2016-02-04)
Protein kinase R (PKR) has previously been suggested to mediate many of the deleterious consequences of a high-fat diet (HFD). However, previous studies have observed substantial phenotypic variability when examining the metabolic consequences of PKR deletion. Accordingly, herein, we have
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实验方案

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神经氨酸酶酶促检测方法

在测定神经氨酸酶活性时，使用了一种以N-乙酰新胺酸为酶底物的比色法进行测定。神经氨酸酶是一种可促进流感病毒从感染细胞中释放的水解酶。

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