A6782
抗-小鼠IgG(全分子)–过氧化物酶 绵羊抗
affinity isolated antibody, buffered aqueous solution
别名:
SAM
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关于此项目
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
46
biological source
sheep
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
小鼠IgG是一种由其重链所定义并来自血浆B细胞的抗体同种型。IgG是在小鼠血清中发现的丰度最高的抗体同种型。IgG可穿过胎盘屏障，成为一种补体激活剂并与吞噬细胞上的Fc受体结合。IgG的水平可能会随疾病或感染的状态而变化。
辣根过氧化物酶（HRP）是一种可催化显色底物转化的酶，如邻苯二胺（OPD）、4-氯-1-萘酚3,3'、5,5'-四甲基联苯胺（TMB）、3,3'′-二氨基联苯胺（DAB）或2,2′-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）；化学发光底物如CPS-3（增强的腔）和荧光底物如Ampliflu™ Red分别成为可检测的发色团、发光体或荧光剂。
辣根过氧化物酶（HRP）是一种可催化显色底物转化的酶，如邻苯二胺（OPD）、4-氯-1-萘酚3,3'、5,5'-四甲基联苯胺（TMB）、3,3'′-二氨基联苯胺（DAB）或2,2′-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）；化学发光底物如CPS-3（增强的腔）和荧光底物如Ampliflu™ Red分别成为可检测的发色团、发光体或荧光剂。
Application
绵羊抗小鼠IgG（全分子）-过氧化物酶多克隆抗体可用于通过显色、化学发光或荧光免疫化学或免疫组织化学技术检测和定量测定小鼠血清和生物体液中的IgG水平。 在在使用小鼠IgG作为一抗的检测中，它还可用作二抗。从山羊白膜层中分离的原代T细胞已使用偶联了HRP的绵羊抗小鼠IgG作为二抗通过western blot进行了分析。
Biochem/physiol Actions
绵羊多克隆抗-小鼠IgG（全分子）-过氧化物酶抗体在体外 、小鼠血清和生物体液中可与小鼠IgG反应 。它不会与人血清蛋白发生反应。
Physical form
0.01 M磷酸盐缓冲盐溶液，pH 7.4，含有1％牛血清白蛋白和防腐剂。
Preparation Note
采用 Wilson,M.B. 和 Nakane,P.K. 在《免疫荧光及相关染色技术》(Immunofluorescence and Related Staining Techniques) 中描述的过碘酸盐法制备（Elsevier/North Holland Biomedical Press 出版社，Amsterdam,p215 (1978)）。
Other Notes
与人血清蛋白吸附的抗体。
Legal Information
Ampliflu is a trademark of Sigma-Aldrich Co. LLC
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
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R A Hederer et al.
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Karin Dietrich et al.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A6782-.5ML | 04061835805518 |
| A6782-1ML | 04061833382837 |
| A6782-.25ML | 04061835805501 |
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