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关于此项目
化学文摘社编号:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, blood typing: suitable, ligand binding assay: suitable
Impurities:
essentially globulin free
Concentration:
≥95% protein
biological source
bovine
Quality Level
assay
≥98%
form
lyophilized powder
purified by
heat shock fractionation
origin
USA origin
concentration
≥95% protein
free fatty acid content
≤0.02%
technique(s)
ELISA: suitable, blood typing: suitable, ligand binding assay: suitable
impurities
essentially globulin free
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
protease ≤0.005 U/mg
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
牛血清白蛋白是一种在调节血液胶体渗透压中起关键作用的蛋白质。一种由583个氨基酸构成的水溶性蛋白,BSA的计算分子量为66,430 道尔顿。六个α-螺旋形成了BSA的三个同源结构域。BSA可依赖于pH进行可逆的构象异构化。蛋白的天然结构在加热时会变得具有反应性和灵活性。
该 BSA 产品针对以下特性经过专门评估和测试:
无蛋白酶 BSA 特别适用于以下应用:
b. 核酸杂交
c. 放射免疫测定(RIA)
该 BSA 产品针对以下特性经过专门评估和测试:
- 非常低的蛋白酶含量/无蛋白酶
- 非常低的脂肪酸含量/无脂肪酸
无蛋白酶 BSA 特别适用于以下应用:
- 酶测定
- 蛋白质测定
- 蛋白酶敏感型技术,包括:
b. 核酸杂交
c. 放射免疫测定(RIA)
Application
牛血清白蛋白已用于:
这种 A7030 BSA 专门用作基于肽的 ELISA 测定中的封闭缓冲液成分,用于分析 COVID-19 / SARS-CoV-2 样品(Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020))。
- 作为人多能干细胞分化培养基的成分
- 酶联免疫吸附试验(ELISA)和夹心 ELISA
- 使胎儿皮层细胞具有通透性
这种 A7030 BSA 专门用作基于肽的 ELISA 测定中的封闭缓冲液成分,用于分析 COVID-19 / SARS-CoV-2 样品(Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020))。
Biochem/physiol Actions
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Features and Benefits
- 热休克分级
- 无蛋白酶
- 无脂肪酸
- 几乎不含球蛋白
Preparation Note
热激分级分离法制取
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A7030-100G | 04061835553563 |
| A7030-5G | 04061833425954 |
| A7030-1KG | 04061835553587 |
| A7030-500G | 04061835553594 |
| A7030-100UG-KC | 04061833463338 |
| A7030-50G | 04061835553600 |
| A7030-10G | 04061835553570 |
| A7030-5KG | 04061835540617 |
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