抗-生物素

通过 Ouchterlony 双扩散和免疫电泳与生物素载体蛋白复合物测定特异性和反应性。未观察到与载体蛋白的反应性。

Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.
Goat Anti-Biotin-Alkaline Phosphatase antibody is specific for biotin when tested against biotin-goat IgG, goat IgG, and KLH.

生物素-KLH 结合物

可能取代亲和素以减少非特异性结合。

在一些应用中，生物素化探针与亲和素的定位会产生高背景水平。抗生物素试剂可替代亲和素，以减少非特异性结合。

ELISA Assays using human spleen cells were performed using alkaline phosphatase-conjugated goat anti-biotin as the secondary antibody with incubation for 1.5 hours at room temperature. Purified IgG from K/BxN, BxSB or C57BL/6 mouse serum was analyzed by western blot using alkaline phosphatase-conjugated goat anti-biotin antibody as the secondary.

Goat Anti-Biotin-Alkaline Phosphatase antibody has been used for dot blot assays. The product can also be used for ELISA (1:30,000) and immunohistochemistry (1:50).

Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.