biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
grade
Molecular Biology
clone
polyclonal
form
liquid
mol wt
antigen 47 kDa
species reactivity
Arabidopsis thaliana
technique(s)
western blot: 0.25-0.5 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
Arabidopsis thaliana ... ATMPK6(818982)
General description
在拟南芥中,有20个基因(MPK)编码推定的植物MAPK,根据序列相似性可将其分为四或五类。所有植物MAPK均可归为植物细胞外调节激酶(PERK)亚家族。
Immunogen
与 拟南芥 MPK6(氨基酸 384-395)相应的 C 端相对应的合成肽。该序列对 AtMPK6 具有特异性(在其他 20 种已知 AtMPK 亚型中未发现)。与 AtMPK3 和 AtMPK4 亚型具有有限的同源性(40-50% 的同一性),并且在烟草直系同源 SIPK 中高度保守。
Biochem/physiol Actions
有丝分裂原激活的蛋白激酶(MAPK)级联是真核生物中最保守的信号转导途径之一。MAPK调节多种细胞活动,包括基因表达、细胞增殖、运动、代谢和细胞凋亡。在拟南芥中,推定的功能分配给AtMPK3、AtMPK4和AtMPK6,三种MAPK均与多种应激反应相关,包括氧化应激信号转导、病原体防御、干旱、高盐、寒冷和触碰。
Physical form
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
Disclaimer
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
A Suite of Receptor-Like Kinases and a Putative Mechano-Sensitive Channel Are Involved in Autoimmunity and Plasma Membrane-Based Defenses in Arabidopsis
Zhang Z, et al.
Molecular plant-microbe interactions : MPMI, 30(2), 150-160 (2017)
Emerging MAP kinase pathways in plant stress signalling
Nakagami H, et al.
Trends in Plant Science, 10(7), 339-346 (2005)
Charlotte H Hurst et al.
Plant physiology, 177(2), 522-531 (2018-04-25)
Receptor-like kinases (RLKs) are the largest family of proteins in plants and are responsible for perceiving the vast majority of extracellular stimuli. Thus, RLKs function in diverse processes, including sensing pathogen attacks, regulating symbiotic interactions, transducing hormone and peptide signals
Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases
MAP Kinases in Plant Signal Transduction (2012)
T Vavrdová et al.
Plant methods, 15, 22-22 (2019-03-23)
In the present work, we provide an account of structured illumination microscopy (SIM) imaging of fixed and immunolabeled plant probes. We take advantage of SIM, to superresolve intracellular structures at a considerable z-range and circumvent its low temporal resolution capacity
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