A7310
抗-水通道蛋白2 兔抗
IgG fraction of antiserum, lyophilized powder
别名:
兔抗水通道蛋白2抗体, 抗-水通道蛋白2, 水通道蛋白2检测
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
rat, human
technique(s)
immunohistochemistry: suitable, western blot: 1:200-1:1000 using using Rat Kidney membranes
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... AQP2(359)
mouse ... Aqp2(11827)
rat ... Aqp2(25386)
General description
水通道蛋白2（AQP2）以四聚体的形式存在，其C末端可具有多种构象并结合了两个镉离子。AQP2基因定位于人染色体12q13.12。它与细胞内囊泡有关。
水通道蛋白2（AQP2）是一种用于调节肾连合小管和集尿管中水平衡的水通道蛋白。AQP2由抗利尿激素激活，然后促进水分流入肾细胞。该蛋白还与整合素相互作用，以调节上皮细胞形态和细胞运动。抗水通道蛋白2抗体通过免疫印迹法识别大鼠中的水通道蛋白2。该抗体也与小鼠和人的AQP2发生反应。
Immunogen
对应于大鼠或小鼠水孔蛋白2（具有额外的N端赖氨酸和酪氨酸）的氨基酸254-271的合成肽通过戊二醛与KLH偶联。该表位在大鼠和小鼠中相同，在人类中高度保守。
Application
使用浓度为5μg/ml的兔抗AQP2对10％福尔马林固定、石蜡包埋的大鼠肾脏切片进行免疫组织化学检测。使用抗AQP2可以观察到髓质粗大的升肢和集合管。
抗-水通道蛋白2兔抗可用于：
- 免疫染色
- 免疫组织化学
- 免疫荧光
- 免疫印迹
抗水通道水孔蛋白2抗体也适用于化学发光蛋白印迹（1：200-1：1000）和免疫印迹。
Biochem/physiol Actions
水通道蛋白（AQP2）N末端区域在运输中起着关键作用。C端磷酸化对根尖膜的透水功能至关重要。此外，在第270位赖氨酸的泛素化对于AQP2实现在溶酶体中的分类和降解，或通过外泌体实现消除是必需的。AQP2基因突变与先天性肾源性尿崩症（NDI）这种水平衡紊乱有关。AQP2的功能失调与先兆子痫、充血性心力衰竭以及肝硬化有关。
Physical form
从含有磷酸盐缓冲液（pH 7.4）、1％BSA和0.05％叠氮化钠的溶液中冻干。
Preparation Note
分离于固定化蛋白A
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
常规特殊物品
常规特殊物品
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