A7554
Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse
clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution
别名:
Monoclonal Anti-Bovine IgG
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关于此项目
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
BG-18, monoclonal
Application:
DB, ELISA (d), IHC (p), WB CL
Citations:
11
biological source
mouse
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
BG-18, monoclonal
form
buffered aqueous glycerol solution
species reactivity
bovine (non-reactive to IgM)
should not react with
pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat
technique(s)
direct ELISA: 1:25,000, dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG, western blot (chemiluminescent): 1:100,000
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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General description
Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Immunogen
Purified bovine IgG
Application
Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- dot blot
- immunohistochemistry
Biochem/physiol Actions
Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.
bovine IgG1 and IgG2
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
低风险生物材料
此项目有
Haet Sal Jeon et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 27(3), 344-351 (2015-04-10)
Antibody responses are useful indicators of Mycobacterium bovis infection in cattle. Many studies have evaluated the ability of immunoglobulin G (IgG) to serodiagnose bovine tuberculosis (TB). In the current study, immunoglobulin A (IgA) and IgG responses against the MPB70 and
Pamela Nicholson et al.
Anaerobe, 56, 78-87 (2019-02-17)
Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the
Pamela Nicholson et al.
Anaerobe, 56, 78-87 (2019-02-17)
Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the
Genetic and immunologic analyses of PlpE, a lipoprotein important in complement-mediated killing of Pasteurella haemolytica serotype 1
Pandher K, et al.
Infection and Immunity, 66(12), 5613-5619 (1998)
Nasrin Husseini et al.
Translational animal science, 6(1), txac006-txac006 (2022-03-10)
The high immune response (HIR) methodology measures the genetic performance of the adaptive immune system to identify and breed animals with balanced and robust immunity. The HIR methodology has previously been used in dairy and swine to reduce disease but
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