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化学文摘社编号:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥96%
Form:
lyophilized powder
Technique(s):
blood typing: suitable
Impurities:
BT and VSV virus, tested
biological source
bovine
Quality Level
assay
≥96%
form
lyophilized powder
quality
suitable for RIA
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
blood typing: suitable
impurities
BT and VSV virus, tested
loss
≤5%
pH
5.2
solubility
water: soluble (40 mg/ml)
suitability
suitable for insulin RIA procedures
UniProt accession no.
foreign activity
BT virus, none detected, VSV virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
牛血清白蛋白(BSA)是一种由每个均带有两个亚结构域的三个结构域所组成的66 kDa蛋白。它是一种α-螺旋、球状且非糖基化的蛋白。
Application
牛血清白蛋白已用于:
- 酶联免疫吸附试验(ELISA)的封闭剂(154)
- 作为窦前卵泡培养的Dulbecco PBS培养基的组分(155)
- 作为卵母细胞授精的改良Tris缓冲培养基(mTBM)的组分(156)
Biochem/physiol Actions
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
牛血清白蛋白(BSA)是一种药物、激素和脂肪酸的转运蛋白。它可在酶联免疫吸附测定(ELISA)中可用作封闭剂以防止抗原和抗体与微量滴定板的非特异性结合。BSA的半胱氨酸和酪氨酸残基对金属结合具有很高的亲和力。
Features and Benefits
- 热休克分级
- 适用于放射免疫分析(RIA)
Preparation Note
使用热休克分级制备
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
Disclaimer
在某些程序中,可能会产生高达0.1 μ单位/毫克的胰岛素空白。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
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