产品名称
抗- 人IgG (γ-链特异性)−过氧化物酶 山羊抗, IgG fraction of antiserum, buffered aqueous solution
biological source
goat
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:40,000
dot blot: 1:100,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Biochem/physiol Actions
This antibody is specific for for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones lambda myeloma proteins.
Immunoglobulin G (IgG) mainly participates in hypersensitivity type II and type III.
Application
Anti-Human IgG (γ-chain specific) Peroxidase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA) and western blot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
IgM concentration in sera from patients with chronic CA syndrome was determined by ELISA using HRP-conjugated goat anti-human (mu chain specific) as the secondary antibody.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . The specificity of Anti-Human IgG (γ-chain specific)-Peroxidase antibody is determined by immunoelectrophoresis and Ouchterlony Double Diffusion techniques. This antibody is specific for for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones lambda myeloma proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.
Immunogen
Human IgG
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
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Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Qingbo Liu et al.
Journal of virology, 95(12) (2021-04-09)
Broadly neutralizing antibodies (bNAbs) are the focus of increasing interest for human immunodeficiency virus type 1 (HIV-1) prevention and treatment. Although several bNAbs are already under clinical evaluation, the development of antibodies with even greater potency and breadth remains a
Qingbo Liu et al.
Nature communications, 10(1), 721-721 (2019-02-15)
Broadly neutralizing antibodies (bNAbs) represent a promising alternative to antiretroviral drugs for HIV-1 prevention and treatment. Selected antibodies to the CD4-binding site bolster envelope trimer binding via quaternary contacts. Here, we rationally engraft a new paratope, i.e., the extended heavy-chain
Heriberto Caballero-Ortega et al.
Journal of infection in developing countries, 8(5), 642-647 (2014-05-14)
There are few articles on evaluation of Toxoplasma gondii serological tests. Besides, commercially available tests are not always useful and are expensive for studies in open population. The aim of this study was to evaluate in-house ELISA and western blot
Philippe V Jutras et al.
PloS one, 11(11), e0167086-e0167086 (2016-11-29)
The overall quality of recombinant IgG antibodies in plants is dramatically compromised by host endogenous proteases. Different approaches have been developed to reduce the impact of endogenous proteolysis on IgGs, notably involving site-directed mutagenesis to eliminate protease-susceptible sites or the
Theodore Kouo et al.
Cancer immunology research, 3(4), 412-423 (2015-02-19)
Galectin-3 is a 31-kDa lectin that modulates T-cell responses through several mechanisms, including apoptosis, T-cell receptor (TCR) cross-linking, and TCR downregulation. We found that patients with pancreatic ductal adenocarcinoma (PDA) who responded to a granulocyte-macrophage colony-stimulating factor-secreting allogeneic PDA vaccine
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