biological source
rabbit
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
bovine
technique(s)
direct ELISA: 1:20,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
免疫球蛋白G(IgG)占血浆蛋白的10−20%,它是最主要的血清蛋白之一。它包含四个亚类:IgG1、IgG2、IgG3和IgG4。IgG结构有四条多肽链,包括两条相同的γ重链(H)和两条相同的κ或λ轻链(L),分别为50 kDa和25 kDa。
Immunogen
纯化牛 IgG
Application
兔抗牛IgG(全分子)-过氧化物酶抗体可用于免疫印迹和ELISA应用。此抗体也可用于斑点印迹(1:80,000)和免疫组化(1:1,000)分析。
兔源抗牛IgG(全分子)过氧物酶抗体可用于:
- 斑点酶联免疫吸附试验 (ELISA)
- 免疫印迹
- 免疫组织学
Biochem/physiol Actions
牛IgG是调节牛群免疫反应的糖蛋白抗体。接种天然H7鞭毛蛋白后,这种抗体可抑制TLR5活化。牛IgG水平可用于检测约内氏病(Johne′s disease )和牛奶掺假。
Physical form
溶于0.01 M磷酸盐缓冲盐水,pH 7.4,含有0.05% MIT
Preparation Note
用两步戊二醛法制备(Avrameas, S. 等人,Scand. J. Immunol.,8,Suppl. 7,7 (1978))。
Disclaimer
除非我们的目录或随附于产品的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG
Baek KH, et al.
Korean journal for food science of animal resources, 34(3), 339-339 (2014)
Comparison of crude and excretory/secretory antigens for the diagnosis of Fasciola hepatica in sheep by western blotting.
Kara, M. and Kircali, F.
Turkish Journal of Veterinary and Animal Sciences, 28, 943-949 (2004)
Investigation of antigenic specificity against Cysticercus tenuicollis cyst fluid antigen in dogs experimentally infected with Taenia hydatigena
Kara M and Douganay A
Turkish Journal of Veterinary and Animal Sciences, 29, 835-840 (2005)
Ingrid Ieda Fernando de Souza et al.
The Journal of veterinary medical science, 81(1), 9-14 (2018-10-12)
Bovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to
George C Russell et al.
Journal of virological methods, 299, 114329-114329 (2021-10-16)
The minor capsid protein of ovine herpesvirus 2, identified as a potential antigen for serological testing, was over-expressed and purified to allow its assessment in ELISA. The corresponding gene sequence (OvHV-2 orf65, Ov65) was modified to incorporate epitope tags and
相关内容
抗体的质量决定了整个免疫检测的成败, 是在选择抗体时首选的考虑因素。基于Sigma®、Chemicon®、Upstate®、Mil l ipore®、 Calbiochem®和Novagen®等子品牌的强强联合,默克在创新的 免疫原设计、免疫、挑选、筛选和验证等方面有着数十年的经验, 创造出许多被广泛使用的抗体,如4G10,Neun,组蛋白修饰等抗 体都是相关领域的“金标”抗体。 我们的技术和科研背景使默克成为高质量抗体的主要设计者和生产 者,而不仅仅是经销商。毋庸置疑,我们的每个小瓶中的抗体都包 含着大量的科学研究成果。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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