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A9045

Sigma-Aldrich

低凝胶温度的琼脂糖

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

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别名:
2-羟乙基琼脂糖
CAS号:
39346-81-1
MDL编号:
MFCD00081294
NACRES:
NA.21

产品线

BioReagent

质量水平

200

形式

powder

技术

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

EEO

≤0.1

mp

≤65 °C ( at a 1.5% gel)

转变温度

congealing temperature 26-30 °C

凝胶强度

≥200 g/cm2 (1% gel)

溶解性

H2O: 10 mg/mL (with heat)

痕量阴离子

sulfate (SO42-): ≤0.10%

异质活性

RNase and DNase free

相关类别

一般描述

琼脂糖是一种藻类多糖。琼脂糖是一种热可逆的离子依赖性胶凝剂。琼脂糖凝胶电泳可用于血浆和其他体液中蛋白质的临床常规分析。它是一种低凝胶温度衍生物，具有独特的胶凝性能。凝胶可在<30°C时形成，在超过60°C的温度下重熔。凝胶表现出优异的透明度，特别适用于制备含有热不稳定材料的培养基。

应用

琼脂糖是一种低凝胶温度衍生物，适用于：

  • 植物细胞培养研究
  • 昆虫细胞培养研究
  • 细胞培养研究

它可用于以下研究：
  • 电泳分离后对确定的RNA和DNA片段进行回收。
  • 细胞化学染色步骤可用于研究排卵前小鼠卵母细胞中的琥珀酸脱氢酶（SDH）的活性。
  • 通过电泳对秀丽隐杆线虫中的RNA进行纯化。

分析说明

以下是与我们的琼脂糖相关的属性列表:
含硫量 -用作纯度指标，因为硫酸盐是主要的离子基团。
凝胶强度 -必须施加于凝胶上使其断裂的力。
凝胶点 -液体琼脂糖溶液冷却时形成凝胶的温度。 琼脂糖溶液在液体至凝胶转变过程中表现出迟滞现象，即凝胶点与熔融温度不同。
电内渗(EEO) -液体通过凝胶的运动。琼脂糖凝胶中的阴离子基团附着在基体上不能移动，但游离的反阳离子可以向基体的阴极迁移，从而产生EEO。 由于生物聚合物的电泳运动通常是向阳极方向的，由于EEO的内部对流，EEO可以干扰这些分离。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I use Agarose, low gelling temperature?

    This product forms a suspension in nearly any aqueous buffer. Heating will melt the agar, which, upon cooling, will form a gel.

  4. Can Agarose, low gelling temperature, be autoclaved?

    This agarose can be autoclaved.

  5. What is the gel strength of Agarose, low gelling temperature?

    We have a specification of not less than 200 g/cm2 for a 1.0% agarose gel.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

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Patrick M Ferree et al.
Scientific reports, 9(1), 12194-12194 (2019-08-23)
Males of hymenopteran insects, which include ants, bees and wasps, develop as haploids from unfertilized eggs. In order to accommodate their lack of homologous chromosome pairs, some hymenopterans such as the honeybee have been shown to produce haploid sperm through
Katherine A Pillman et al.
The EMBO journal, 37(13) (2018-06-07)
Members of the miR-200 family are critical gatekeepers of the epithelial state, restraining expression of pro-mesenchymal genes that drive epithelial-mesenchymal transition (EMT) and contribute to metastatic cancer progression. Here, we show that miR-200c and another epithelial-enriched miRNA, miR-375, exert widespread
Nariko Arimura et al.
Science advances, 6(36) (2020-09-13)
For normal neurogenesis and circuit formation, delamination of differentiating neurons from the proliferative zone must be precisely controlled; however, the regulatory mechanisms underlying cell attachment are poorly understood. Here, we show that Down syndrome cell adhesion molecule (DSCAM) controls neuronal
A Fire et al.
Nature, 391(6669), 806-811 (1998-03-05)
Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an endogenous gene. Such effects have been proposed to result from a simple antisense mechanism that depends on hybridization between the
Agarose gel electrophoresis.
Johansson B G.
Scandinavian Journal of Clinical and Laboratory Investigation, 29(S124), 7-19 (1972)
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