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Biological source:
bovine
Assay:
28-32% protein basis (biuret)
Form:
solution
Technique(s):
ELISA: suitable, electrophoresis: suitable, western blot: suitable
Impurities:
≤0.01% free fatty acid
Concentration:
28-32% protein (biuret), 30% in saline
biological source
bovine
Quality Segment
sterility
aseptically filled
assay
28-32% protein basis (biuret)
form
solution
purified by
cold ethanol fractionation
does not contain
stabilizer or preservative
origin
USA origin
concentration
28-32% protein (biuret), 30% in saline
free fatty acid content
≤0.01%
technique(s)
ELISA: suitable, electrophoresis: suitable, western blot: suitable
impurities
≤0.01% free fatty acid
UniProt accession no.
foreign activity
BT virus, none detected, VSV virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
牛血清白蛋白(BSA)具有三个同源结构域,每个结构域将其细分为两个结构域(A和B)。它是一种α-螺旋球状蛋白,与人血清白蛋白具有相同的结构特征。
Application
牛血清白蛋白溶液已用于:
- 作为Dulbecco′s改良Eagle′s培养基的补充剂用于培养HepG2人肝癌症细胞
- 作为肝细胞复苏培养基的组分
- 作为绵羊体外复溶胚胎的融合和活化缓冲液的组分
Biochem/physiol Actions
牛血清白蛋白(BSA)是血液中主要的蛋白,具有钙结合位点并调节金属稳态。它是细胞培养基的一种重要组成部分,可促进胚胎干细胞(hESC)的分化。由于其具有高溶解度、低成本和纯度等优点,被广泛应用于化妆品和医药工业。 BSA在酶联免疫吸附测定(ELISA)中可用作封闭剂以防止抗原和抗体与微量滴定板的非特异性结合。
Preparation Note
采用冷乙醇沉淀分离法进行初次分级分离
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)