A9357
Anti-alpha-Actin Antibody, Cardiac Muscle (ACTC1) Antibody
mouse monoclonal, AC1-20.4.2
别名:
Anti-ACTC, Anti-ACTC1, Anti-Actin alpha cardiac muscle 1, Anti-CMD1R, Anti-CMH11
产品名称
Anti-Actin, Cardiac antibody, Mouse monoclonal, clone AC1-20.4.2, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC1-20.4.2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa
species reactivity
rat, human, bovine, chicken, mouse
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using digested rat heart tissue
indirect ELISA: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTC1(70)
mouse ... Actc1(11464)
rat ... Actc1(29275)
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Actin, Cardiac antibody is suitable for use in immunocytochemistry, immunoblot (approx. 42 kDa), indirect ELISA, and western blot. The antibody may also be used in immunohistochemistry (using formalin-fixed, paraffin-embedded sections, or using a conc. of 10-20 μg/mL in digested rat heart tissue).
Protein samples isolated from cardiac sections or cardiac myocytes were subjected to western blot using monoclonal mouse anti-actin antibody as the primary at a dilution of 1:2000.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Actins are cytoskeletal proteins that are widely expressed in eukaryotes. These proteins primarily regulate various cellular functions such as muscle contraction, mRNA synthesis and apoptosis among many others. ACTC1 is the predominant actin isoform form in adult cardiac tissues and alterations in this isoform have been associated with hypertrophic cardiomyopathy and congenital heart disease . Monoclonal Anti-Actin, Cardiac antibody is specific for human, bovine, chicken, rat, and mouse cardiac α actin.
Immunogen
synthetic NH2-terminal decapeptide of cardiac isoform of actin.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
常规特殊物品
此项目有
W W Franke et al.
Differentiation; research in biological diversity, 60(4), 245-250 (1996-07-01)
We describe three murine monoclonal antibodies (mAbs) raised against a synthetic decapeptide representing the aminoterminal sequence of the cardiac/ fetal isoform of sarcomeric alpha-actin. When used for immunoblotting or histological immunolocalization, these mAbs distinguish cardiac/fetal alpha-actin from skeletal muscle alpha-actin
Hong-Kun Jiang et al.
Circulation journal : official journal of the Japanese Circulation Society, 74(11), 2410-2418 (2010-10-22)
The Cardiac α actin 1 gene (ACTC1) has been related to familial atrial septal defects. This study was set to explore a potential role of this gene in the formation of sporadic congenital heart disease (CHD). Assessment of cardiac tissue
Pratik A Lalit et al.
Nature protocols, 12(5), 1029-1054 (2017-04-21)
Here we describe a protocol to generate expandable and multipotent induced cardiac progenitor cells (iCPCs) from mouse adult fibroblasts using forced expression of Mesp1, Tbx5, Gata4, Nkx2.5 and Baf60c (MTGNB) along with activation of Wnt and JAK/STAT signaling. This method
Nadia Rachdaoui et al.
Journal of the Endocrine Society, 3(1), 69-90 (2019-01-31)
Chronic hyperinsulinemia, in vivo, increases the resistance of peripheral tissues to insulin by desensitizing insulin signaling. Insulin, in a heterologous manner, can also cause IGF-1 resistance. The aim of the current study was to investigate whether insulin-mediated insulin and IGF-1
Iel-Yong Sung et al.
International journal of medical sciences, 13(11), 841-852 (2016-11-24)
The purpose of the present study was to investigate the in vitro cardiomyogenic differentiation potential of human dental follicle-derived stem cells (DFCs) under the influence of suberoylanilide hydroxamic acid (SAHA), a member of the histone deacetylase inhibitor family, and analyze
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