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A9357

Anti-alpha-Actin Antibody, Cardiac Muscle (ACTC1) Antibody

Name: Anti-alpha-Actin Antibody, Cardiac Muscle (ACTC1) Antibody
Brand: SIGMA

mouse monoclonal, AC1-20.4.2

别名:

Anti-ACTC, Anti-ACTC1, Anti-Actin alpha cardiac muscle 1, Anti-CMD1R, Anti-CMH11

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关于此项目

UNSPSC Code:

12352203

NACRES:

NA.41

Conjugate:

unconjugated

Clone:

AC1-20.4.2, monoclonal

Application:

ELISA (i), ICC, IHC (p), WB

Citations:

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属性

产品名称

Anti-Actin, Cardiac antibody, Mouse monoclonal, clone AC1-20.4.2, purified from hybridoma cell culture

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC1-20.4.2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

rat, human, bovine, chicken, mouse

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using digested rat heart tissue, indirect ELISA: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

P68032

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTC1(70)
mouse ... Actc1(11464)
rat ... Actc1(29275)

说明

General description

Actins are cytoskeletal proteins that are widely expressed in eukaryotes. These proteins primarily regulate various cellular functions such as muscle contraction, mRNA synthesis and apoptosis among many others. ACTC1 is the predominant actin isoform form in adult cardiac tissues and alterations in this isoform have been associated with hypertrophic cardiomyopathy and congenital heart disease . Monoclonal Anti-Actin, Cardiac antibody is specific for human, bovine, chicken, rat, and mouse cardiac α actin.

Immunogen

synthetic NH₂-terminal decapeptide of cardiac isoform of actin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Monoclonal Anti-Actin, Cardiac antibody is suitable for use in immunocytochemistry, immunoblot (approx. 42 kDa), indirect ELISA, and western blot. The antibody may also be used in immunohistochemistry (using formalin-fixed, paraffin-embedded sections, or using a conc. of 10-20 μg/mL in digested rat heart tissue).

Protein samples isolated from cardiac sections or cardiac myocytes were subjected to western blot using monoclonal mouse anti-actin antibody as the primary at a dilution of 1:2000.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

wgk

WGK 1

法规信息

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低风险生物材料

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000426486

0000397426

0000251263

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全球贸易项目编号

货号	GTIN
A9357-200UL	04061833402382
A9357-100UL	04061837718984