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Biological source:
bovine
Assay:
≥96% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Impurities:
BT and VSV virus, tested
biological source
bovine
product line
BioReagent
assay
≥96% (agarose gel electrophoresis)
form
lyophilized powder
Quality Level
origin
USA origin
technique(s)
cell culture | mammalian: suitable
impurities
BT and VSV virus, tested
loss
≤5%
pH
5.0-5.6
solubility
H2O: 40 mg/mL
foreign activity
BT virus, none detected, VSV virus, none detected
shipped in
ambient
storage temp.
2-8°C
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General description
牛血清白蛋白是各种研究中的模型蛋白,由 583 个氨基酸组成。它是一种水溶性蛋白质,分子量为66.4kDa。六个α-螺旋形成了BSA的三个同源结构域。它可依赖于pH进行可逆的构象异构化。蛋白的天然结构在加热时会变得具有反应性和灵活性。
Application
牛血清白蛋白已用于流式细胞仪分析的洗涤液、组织消化液和 ELISA(酶联免疫吸附试验)的封闭液。它也被用于流式细胞仪的缓冲液重悬。
Biochem/physiol Actions
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
牛血清白蛋白被广泛用作细胞培养基,特别是无血清培养基的添加剂。它可带来一系列的好处,包括防止氧化损伤和稳定其他培养基成分,如脂肪酸和吡哆醛。
Preparation Note
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
通常称为第五组分(Cohn fraction V);本品采用改进的Cohn氏冷乙醇分级分离法制备。
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
存储类别
13 - Non Combustible Solids
法规信息
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Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.
本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能,并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。这些深度知识可能也适用于离体和组织工程学应用。
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