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Merck
CN

A9543

牛血清白蛋白 来源于牛血清

heat shock fraction, low endotoxin, pH 7, ≥98%

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
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产品名称

牛血清白蛋白 来源于牛血清, heat shock fraction, low endotoxin, pH 7, ≥98%

biological source

bovine

assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

impurities

≤1.0 EU/mg endotoxin

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

foreign activity

BT virus, none detected
VSV virus, none detected

storage temp.

2-8°C

Quality Level

Gene Information

bovine ... ALB(280717)

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Biochem/physiol Actions

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

Preparation Note

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
Prepared using heat shock fractionation

Application

This A9543 BSA product has been used in various systems, including:
  • NRK-52E rat kidney epithelioid cells: Ochiai, H. et al., J. Virol., 67(11), 6811-6814 (1993)
  • Neonatal porcine pancreatic islets: Ellis, C.E. et al., Islets, 5(5), 216-225 (2013)
  • Mouse:
Lee, D. et al., Am. J. Physiol. Renal Physiol., 304(7), F1009-F1019 (2013)
Girbl, T. et al., Immunity, 49(6), 1062-1076.e6 (2018)
  • iPSC cell line: Martin, H.C. et al., Science, 362(6419), 1161-1164 (2018).

Features and Benefits

  • Heat shock fractionated
  • Low endotoxin

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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Anna Barkaway et al.
Immunity, 54(7), 1494-1510 (2021-05-26)
Aging is associated with dysregulated immune functions. Here, we investigated the impact of age on neutrophil diapedesis. Using confocal intravital microscopy, we found that in aged mice, neutrophils adhered to vascular endothelium in inflamed tissues but exhibited a high frequency
Natalia Reglero-Real et al.
Immunity, 54(9), 1989-2004 (2021-08-08)
The migration of neutrophils from the blood circulation to sites of infection or injury is a key immune response and requires the breaching of endothelial cells (ECs) that line the inner aspect of blood vessels. Unregulated neutrophil transendothelial cell migration
Balázs Rada et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4883-4893 (2008-09-20)
The dual oxidase-thiocyanate-lactoperoxidase (Duox/SCN(-)/LPO) system generates the microbicidal oxidant hypothiocyanite in the airway surface liquid by using LPO, thiocyanate, and Duox-derived hydrogen peroxide released from the apical surface of the airway epithelium. This system is effective against several microorganisms that
Marcos V A S Navarro et al.
PLoS biology, 9(2), e1000588-e1000588 (2011-02-10)
The bacterial second messenger bis-(3'-5') cyclic dimeric guanosine monophosphate (c-di-GMP) has emerged as a central regulator for biofilm formation. Increased cellular c-di-GMP levels lead to stable cell attachment, which in Pseudomonas fluorescens requires the transmembrane receptor LapD. LapD exhibits a
Ignacio Izeddin et al.
PloS one, 6(1), e15611-e15611 (2011-01-26)
The actin cytoskeleton of dendritic spines plays a key role in morphological aspects of synaptic plasticity. The detailed analysis of the spine structure and dynamics in live neurons, however, has been hampered by the diffraction-limited resolution of conventional fluorescence microscopy.

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