A9792
抗-鸡 IgY（IgG）（全分子）−过氧化物酶 兔抗
IgG fraction of antiserum, buffered aqueous solution
别名:
Rabbit Anti-Chicken IgY (IgG) (whole molecule)−HRP
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关于此项目
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p)
Citations:
8
biological source
rabbit
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
chicken
technique(s)
direct ELISA: 1:30,000, dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate., immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
鸡 IgY 由两条重链（H）和两条轻链（L）组成。其分子量约为 180 kDa。
Immunogen
纯化鸡IgY(IgG)
Application
以HRP标记的兔抗鸡IgY（1:2000稀释液）作为二抗，通过蛋白质免疫印迹法分析禽类蛋白（大肠杆菌过表达蛋白纯化制品）。
兔抗-鸡IgY(IgG)（全分子）-过氧化物酶抗体可用于ELISA分析。此抗体还可用于斑点印迹(1:160,000)和IHC(1:500)分析。
抗-鸡IgY(IgG)（全分子）FITC抗体已用于免疫组织学分析。
Biochem/physiol Actions
IgY在抵抗系统性感染的防御机制中起着重要作用。它还能引发过敏反应。
兔抗鸡IgY(IgG)（全分子）-过氧化物酶抗体结合鸡IgY。
鸡IgY是鸡血清中的主要抗体。鸡对哺乳动物抗原蛋白产生相对增强的抗体反应。这些免疫球蛋白不与哺乳动物和细菌中的Fc受体结合，也不与类风湿因子相互作用。因此，此抗鸡抗体是各种研究应用中重要的分析工具。
Physical form
溶于0.01M磷酸缓冲盐，pH7.4，含有0.05%MIT。
Preparation Note
通过Avrameas, S., et al., Scand.J. Immunol., 8, Suppl. 7, 7 (1978)等描述的两步戊二醛方法进行制备。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
N Obermüller et al.
American journal of physiology. Renal physiology, 280(2), F244-F253 (2001-02-24)
Because proteinuria has been demonstrated in patients with autosomal-dominant polycystic kidney disease (ADPKD), we have investigated whether proteinuria also occurs in the (cy/+) rat, a widely used model for ADPKD. Increased urinary excretion of proteins, in particular of albumin, can
G Haqshenas et al.
The Journal of general virology, 83(Pt 9), 2201-2209 (2002-08-20)
We recently identified a novel virus, designated avian hepatitis E virus (avian HEV), from chickens with hepatitis-splenomegaly (HS) syndrome in the USA. We showed that avian HEV is genetically related to swine and human HEVs. Here we report the antigenic
Energetic stress, immunosuppression and the costs of an antibody response.
Svensson, E., et al.
Functional Ecology, 12(6), 912-919 (1998)
IgY: clues to the origins of modern antibodies
Warr G W, et al.
Immunology Today, 16(8), 392-398 (1995)
John C Fyfe et al.
Neurogenetics, 12(3), 223-232 (2011-06-07)
We recently reported autosomal recessive fetal-onset neuroaxonal dystrophy (FNAD) in a large family of dogs that is not caused by mutation in the PLA2G6 locus (Fyfe et al., J Comp Neurol 518:3771-3784, 2010). Here, we report a genome-wide linkage analysis
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