Merck
CN

AMPD1

Sigma-Aldrich

脱氧核糖核酸酶I

Amplification Grade

登录to View Organizational & Contract Pricing

Select a Size

Change View
别名:
脱氧核糖核酸酶 I(DNA酶I)
NACRES:
NA.55

质量水平

形式

liquid

浓度

1 unit/μL

technique(s)

RT-PCR: suitable

颜色

colorless

运输

wet ice

储存温度

−20°C

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
D531910104159001DNASE70
DNase I Amplification Grade

Sigma-Aldrich

AMPD1

脱氧核糖核酸酶I

Deoxyribonuclease I bovine recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, ≥5,000 units/mg protein

Sigma-Aldrich

D5319

脱氧核糖核酸酶 I 牛

DNase I grade II, from bovine pancreas

Roche

10104159001

DNase I

concentration

1 unit/μL

concentration

-

concentration

-

concentration

-

technique(s)

RT-PCR: suitable

technique(s)

-

technique(s)

DNA extraction: suitable

technique(s)

RNA purification: suitable

color

colorless

color

-

color

-

color

-

shipped in

wet ice

shipped in

dry ice

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

一般描述

脱氧核糖核酸酶I(DNase I)是一种从牛胰腺中分离的内切核酸酶,可将双链和单链DNA消化成寡核苷酸和单核苷酸。 扩增级 DNase I 已纯化去除RNase活性,适于在 RT-PCR(逆转录酶 - 聚合酶链反应)等敏感应用之前去除 RNA 制剂中的DNA。

DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液,在室温下消化 15 分钟,可去除 RNA 制剂中的 DNA。然后随同终止液加热,使DNase I失活。加热还会使RNA中的发夹变性,可直接用于逆转录。

当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明,Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。

应用

扩增级脱氧核糖核酸酶I(DNase I)已用于:
  • 在 RNA 的分离和纯化过程中消化 DNA。纯化的 RNA 可用于通过RNA 逆转录酶合成 cDNA。
  • 水解细胞外基质 (ECM) 成分并增强光敏剂在生物膜中的渗透以确定抗菌光动力疗法 (aPDT) 对白色念珠菌生物膜的功效
  • 从牛血样中提取的总 RNA 中去除污染 DNA

特点和优势

  • 适用于去除 RNA 中的 DNA
  • 超低RNase活性
  • 经过优化的10×反应缓冲液和终止液,可使DNase I完全失活

适用性

适用于去除 RNA 制剂中的 DNA。

单位定义

在 37 °C 下,一个单位可在 10 min 内将 1μg 质粒DNA 消化为寡核苷酸。

法律信息

购买本产品附带有限的许可,仅可用于研究用途聚合酶链式反应 (PCR) 过程,且应配合热循环仪使用。该热循环仪在 PCR 过程的自动执行中的使用由预先许可费涵盖,该费用通过向 Applied Biosystems 支付或购买的方式(即授权的热循环仪)支付。

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of the DNAse in Product AMPD1, DNase I?

    The DNAse is provided at 1 unit/uL in 1 mL total volume.

  4. What do I do if my DNA is not completely digested after 15 minute digestion at room temperature when using Product AMPD1, DNase I?

    If further digestion is required, incubation can be performed for 30 minutes at 37°C.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

John P Dunbar et al.
Toxins, 12(6) (2020-06-24)
The noble false widow spider Steatoda nobilis originates from the Macaronesian archipelago and has expanded its range globally. Outside of its natural range, it may have a negative impact on native wildlife, and in temperate regions it lives in synanthropic
Hafid Ait-Oufella et al.
The Journal of experimental medicine, 207(8), 1579-1587 (2010-07-07)
B cell depletion significantly reduces the burden of several immune-mediated diseases. However, B cell activation has been until now associated with a protection against atherosclerosis, suggesting that B cell-depleting therapies would enhance cardiovascular risk. We unexpectedly show that mature B
Ling Yuan et al.
Molecular vision, 18, 1684-1695 (2012-07-10)
The objectives of this study were to determine whether high-glucose-induced upregulation of heparanase (HPSE) expression and differential heparanase expression in human retinal vascular endothelial cells (HRECs) can alter HREC migration and proliferation. We also aimed to determine whether HREC migration
Hye-Ryoung Kim et al.
Virology journal, 17(1), 53-53 (2020-04-16)
Runting-stunting syndrome (RSS) in chickens, also known as malabsorption syndrome, which is characterized by mild to severe enteritis and diagnosed through typical histopathologic examination as well as clinical signs, results in considerable economic losses. Despite the many studies carried out
Molecular characterization and differential mRNA expression profiling of Toll-like receptor-2 gene in Vechur (Bos indicus) and crossbred (Bos indicus X Bos taurus) cattle of Kerala in response to anthrax vaccination
Shivakumara P N, et al.
Meta Gene, 16, 15-20 (2018)

商品

SeqPlex™-I Whole Transcriptome Amplification Kits for Next-Generation Sequencing

Whole transcriptome amplification (WTA) is a method often used to amplify small quantities of reverse transcribed RNA, or degraded RNA for direct use in Next-Generation Sequencing (NGS) workflows. Discover our SeqPlex™-I WTA library preparation kit for producing libraries that are ready for direct input onto Illumina® NGS flow cells.

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

实验方案

SEQR – SeqPlex RNA扩增试剂盒实验方案

SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法,

Reverse Transcription Protocol

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

逆转录实验方案

将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

相关内容

Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

PCR的应用_热启动PCR_逆转录酶 PCR-默克生命科学

PCR是分子生物学的核心技术,功能强大。可在体外高效快速地对不同来源的特定DNA或RNA序列进行酶扩增。

KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门