产品名称
单克隆抗 FITC-生物素 小鼠抗, clone FL-D6, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FL-D6, monoclonal
form
buffered aqueous solution
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Biochem/physiol Actions
该抗体可与游离的和结合的异硫氰酸荧光素(FITC)反应。
Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.
Immunogen
FITC-BSA偶联物
Application
Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:
- for amplification of signal in immunofluorescence assays
- in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5
- enzyme linked immunosorbent assay (ELISA)
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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Chapter 11 - (Strept)avidin?Biotin Systems
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Arteriosclerosis, thrombosis, and vascular biology, 34(10), 2237-2245 (2014-08-12)
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