B1064
Cibacron Blue 3G-A
Dye content ≥55 %, Powder
别名:
汽巴蓝；1-氨基-4-[4-[[4-氯-6-(2-璜苯胺)-1,3,5-三嗪-2-基]氨基]-3-璜苯胺]-9,10-蒽醌-2-磺酸
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关于此项目
经验公式（希尔记法）:
C29H20ClN7O11S3
化学文摘社编号:
分子量:
774.16
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47
产品名称
Cibacron Blue 3G-A, Dye content ≥55 %
SMILES string
Nc1c(cc(Nc2ccc(Nc3nc(Cl)nc(Nc4ccccc4S(O)(=O)=O)n3)c(c2)S(O)(=O)=O)c5C(=O)c6ccccc6C(=O)c15)S(O)(=O)=O
InChI
1S/C29H20ClN7O11S3/c30-27-35-28(33-16-7-3-4-8-19(16)49(40,41)42)37-29(36-27)34-17-10-9-13(11-20(17)50(43,44)45)32-18-12-21(51(46,47)48)24(31)23-22(18)25(38)14-5-1-2-6-15(14)26(23)39/h1-12,32H,31H2,(H,40,41,42)(H,43,44,45)(H,46,47,48)(H2,33,34,35,36,37)
InChI key
YKCWQPZFAFZLBI-UHFFFAOYSA-N
form
powder
composition
Dye content, ≥55%
color
dark blue
solubility
H2O: 10 mg/mL, blue
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Application
当使用琼脂糖等不溶性多孔支持基质固定时，Cibacron Blue 3GA被用于酶的亲和层析纯化。这种亲和力归因于染料与具有辅因子结合域的蛋白质的天然配体之间的结构相似性。
Biochem/physiol Actions
Cibacron Blue 3GA是一种阴离子蒽醌染料。 它是一种P2-嘌呤受体拮抗剂 并且能抑制刺激诱发的大鼠脑皮质组织的谷氨酸释放。Cibacron Blue 3GA能抑制OXA-1 和OXA-2β-内酰胺酶 ，并被用于通过监测色氨酸荧光观察配体与OXA-1 β-内酰胺酶的结合。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
C Monaghan et al.
The Biochemical journal, 205(2), 413-417 (1982-08-01)
Although beta-lactamases do not require any nucleotide co-substrates, the OXA-2 type is inhibited competitively by Cibacron Blue 3GA, and by other anthraquinone dyes, including some simpler compounds with no side chain. The enzyme causes a red shift in the spectrum
G C Bennett et al.
British journal of pharmacology, 131(3), 617-623 (2000-10-04)
Evidence has previously been presented that P1 receptors for adenosine, and P2 receptors for nucleotides such as ATP, regulate stimulus-evoked release of biogenic amines from nerve terminals in the brain. Here we investigated whether adenosine and nucleotides exert presynaptic control
Kyle D Schneider et al.
Biochemistry, 48(26), 6136-6145 (2009-06-03)
Class D beta-lactamases hydrolyze beta-lactam antibiotics by using an active site serine nucleophile to form a covalent acyl-enzyme intermediate and subsequently employ water to deacylate the beta-lactam and release product. Class D beta-lactamases are carboxylated on the epsilon-amino group of
S T Thompson et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(2), 669-672 (1975-02-01)
A procedure is described to utilize blue dextran-Sepharose as an affinity chromatographic column specific for the super-secondary structure called the dinucleotide fold, which forms the binding sites for substrates and effectors on a wide range of proteins. The procedure can
I von Kügelgen et al.
British journal of pharmacology, 113(3), 815-822 (1994-11-01)
1. Some postganglionic sympathetic axons possess P2Y-like P2-purinoceptors which, when activated, decrease the release of noradrenaline. We examined the question of whether such receptors also occur at the noradrenergic axons in the rat brain cortex. Slices of the brain cortex
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