General description
考马斯亮蓝染色剂在十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS PAGE) 中被广泛用于蛋白质的检测。亮蓝 G 染色因其灵敏度接近银染法而被称为蓝银染色。考马斯亮蓝 G 染色液通过疏水作用与蛋白质结合。它可以有效染色角膜前囊膜，毒性相对较小。
Application
亮蓝 G-胶体浓缩液用于在分析酿酒酵母中的转录因子（TFIID）复合物前，进行体外胃蛋白酶消化后卵巢肿瘤液蛋白质组中
- 的蛋白质片段的染色
Preparation Note
用 800 mL 水稀释至 1 L 后，混悬液将含有 0.1% (w/v) 亮蓝 G、0.29 M 磷酸和 16% 饱和硫酸铵。
Analysis Note
已在 SDS-PAGE 上检测了本品的适用性。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis
Candiano G, et al.
Electrophoresis, 25(9), 1327-1333 (2004)
Proteomic analysis of ovarian cancer tumor fluid is a rich source of potential biomarkers
Poersch A, et al.
Journal of Proteomics and Bioinformatics, 5(2), 2-2 (2014)
Elena Barbieri et al.
Journal of aging research, 2011, 845379-845379 (2011-06-02)
This study describes mitochondrial behaviour during the C2C12 myoblast differentiation program and proposes a proteomic approach to mitochondria integrated with classical morphofunctional and biochemical analyses. Mitochondrial ultrastructure variations were determined by transmission electron microscopy; mitochondrial mass and membrane potential were
A proteomic signature of ovarian cancer tumor fluid identified by highthroughput and verified by targeted proteomics
Poersch A, et al.
Journal of proteomics, 145(2), 226-236 (2016)
Roy Auty et al.
The Journal of biological chemistry, 279(48), 49973-49981 (2004-09-28)
The basal transcription factor TFIID is composed of the TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Although TBP alone binds to the TATA box of DNA and supports basal transcription, the TAFs have essential functions that remain poorly defined.
实验方案
Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| B2025-1EA | 04061833426524 |
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