应用
来自菠萝茎的菠萝蛋白酶已被用于：
- 评估由离子液助表面活性剂组成的双水相胶束系统
- 作为十二烷基硫酸钠（SDS）/聚丙烯酰胺凝胶电泳（PAGE）和细胞增殖试验中的阳性对照
- 研究其潜在的镇痛作用并评估其对含有脑啡肽序列的前脑啡肽（PENK）衍生肽的水解作用
菠萝蛋白酶可用于抑制促炎前列腺素的生物合成。可用于降低凝血效率。来自菠萝茎的菠萝蛋白酶已被用于制备蜜蜂采集的花粉的酶水解产物。
生化/生理作用
菠萝蛋白酶是一种半胱氨酸内肽酶，对蛋白质的裂解具有广泛的特异性。菠萝蛋白酶可能来自干或水果。产品 B4882 来自菠萝茎。产品 P3169（pGlu-Phe-Leu-P-硝基苯胺）可用作菠萝蛋白酶含量测定的显色底物。
制备说明
将本品混悬于 pH 4.5 醋酸盐缓冲液中，浓度为 1 mg/mL，得到浑浊的类白色混悬液。
其他说明
25°C、pH 4.6 下 1 个单位每分钟将从 N-α-CBZ-L-赖氨酸对硝基苯酯中释放 1.0 μmol 对硝基苯酚。1 个旧的滴定单位 (45°C、pH 4.5) 相当于约 1.7 个新单位 (25°C、pH 4.6)。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
此项目有
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente FA, et al.
Process. Biochem., 51(4), 528-534 (2016)
Enkephalin related peptides are released from jejunum wall by orally ingested bromelain
Orlandi-Mattos PE, et al.
Peptides, 115, 32-42 (2019)
In vitro antiproliferative activity of fresh pineapple juices on ovarian and colon cancer cell lines
Gani MBA, et al.
International Journal of Peptide Research and Therapeutics, 21(3), 353-364 (2015)
Soghra Khatun Haq et al.
European journal of biochemistry, 269(1), 47-52 (2002-01-11)
Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.32) were performed by CD spectroscopy, fluorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH 2.0, stem bromelain lacks a well defined
V Grabovac et al.
International journal of pharmaceutics, 326(1-2), 153-159 (2006-09-05)
The aim of this study was to investigate the influence of the proteolytic enzyme bromelain on the permeation of heparin across the gastrointestinal epithelial barrier. Stability of the complex and effect of heparin on the enzymatic activity of bromelain was
实验方案
Protocol for HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column
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