B5144
菠萝蛋白酶 来源于菠萝的茎
≥4 units/mg protein, (chromatography purified)
表单
lyophilized powder
质量水平
比活
(chromatography purified)
≥4 units/mg protein
组成
Protein, 30-50% biuret
溶解性
H2O: soluble 1.0 mg/mL, clear, colorless
储存温度
−20°C
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应用
来自菠萝茎的菠萝蛋白酶已被用作十二烷基硫酸钠（SDS）/聚丙烯酰胺凝胶电泳（PAGE）酶谱中的阳性对照。它还被用作测定蛋白水解活性的偶氮酪蛋白底物。
菠萝蛋白酶可用于抑制促炎前列腺素的生物合成。可用于降低凝血效率。来自菠萝茎的菠萝蛋白酶已被用于制备蜜蜂采集的花粉的酶水解产物。
生化/生理作用
菠萝蛋白酶是一种半胱氨酸内肽酶，对蛋白质的裂解具有广泛的特异性。菠萝蛋白酶可能来自干或水果。产品 P3169（pGlu-Phe-Leu-P-硝基苯胺）可用作菠萝蛋白酶含量测定的显色底物。
外形
含甘露醇和磷酸钾缓冲盐的冻干粉
制备说明
色谱纯化。溶于 1 mg/mL 浓度的水中，形成澄清、无色溶液。
色谱纯化产品
其他说明
25 °C 下 pH 4.6 下，1 个单位每分钟将从 N-α-Z-L-赖氨酸对硝基苯酯中释放 1.0 μmol 对硝基苯酚。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Larisa V Kordyukova et al.
Membranes, 11(10) (2021-10-23)
Influenza A virus envelope contains lipid molecules of the host cell and three integral viral proteins: major hemagglutinin, neuraminidase, and minor M2 protein. Membrane-associated M1 matrix protein is thought to interact with the lipid bilayer and cytoplasmic domains of integral
Acidic cysteine protease inhibitors from pineapple stem.
R L Heinrikson et al.
Methods in enzymology, 45, 740-751 (1976-01-01)
Nora Luniak et al.
Biotechnology progress, 33(1), 54-65 (2016-11-20)
Expression of proteases in heterologous hosts remains an ambitious challenge due to severe problems associated with digestion of host proteins. On the other hand, proteases are broadly used in industrial applications and resemble promising drug candidates. Bromelain is an herbal
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
S Habib et al.
The protein journal, 26(2), 117-124 (2007-01-05)
Trehalose, a naturally occurring osmolyte, is considered as a universal protein stabilizer. We investigated the effect of the disaccharides, trehalose and sucrose, on the thermal stability and conformation of bromelain. To our surprise, bromelain in the presence of 1 M
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