B5388
亮甲酚蓝 ALD
Dye content, ≥60%, certified by the Biological Stain Commission, powder
别名:
3-氨基-7-（二乙氨基）-2-甲基吩恶嗪-5-铵 (T-4)-四氯锌酸盐 (2:1), 亮甲酚蓝, 亮蓝 C
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线性分子式:
C17H20N3OCl · 1/2ZnCl2
化学文摘社编号:
分子量:
385.96
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
279-675-0
MDL number:
Beilstein/REAXYS Number:
5690713
Colour Index Number:
51010
产品名称
亮甲酚蓝 ALD, certified by the BSC
SMILES string
[Zn-2](Cl)(Cl)(Cl)Cl.N(CC)(CC)c4cc5[o+]c6c(nc5cc4)cc(c(c6)N)C.N(CC)(CC)c1cc2[o+]c3c(nc2cc1)cc(c(c3)N)C
InChI key
SIHASHFMDFXGSX-UHFFFAOYSA-J
InChI
1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4
agency
certified by the BSC
form
powder
composition
Dye content, ≥60%
solubility
water: 1 mg/mL, blue to very deep blue
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
Application
灿烂甲酚蓝 ALD 已用于卵母细胞的选择。它也被用于选择卵丘-卵母细胞复合体 (COCs)。
经认证为网织红细胞的活上染色。
Biochem/physiol Actions
灿烂甲酚蓝 ALD 主要用于卵母细胞的选择。它有助于测定葡萄糖-6-磷酸脱氢酶 (G6PD) 的活性。这种酶在生长的卵母细胞中产生。然而，完成生长的卵母细胞 G6PD 水平较低。由于亮甲酚蓝不能还原成无色化合物，所以低 G6PD 活性导致细胞质呈蓝色。它也用于网织红细胞染色，其中染料染色网硬蛋白。
Other Notes
灿烂甲酚蓝 ALD 的结构在吩恶嗪核心上含有 2-甲基修饰，而历史性灿烂甲酚蓝的结构在吩恶嗪核心上含有 8-甲基修饰。两种结构的生物染色应用相同。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Vitrification of immature and matured bovine oocytes: effect of brilliant cresyl blue selection and hyaluronan addition.
Animal Reproduction, 13 (2016)
Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation.
Rodriguez-Gonzalez E, et al.
Reproduction, Nutrition, Development, 43, 179-179 (2003)
Cheesbrough M
District Laboratory Practice in Tropical Countries (2006)
A Zh Baltabekova et al.
Molecular biotechnology, 57(8), 746-755 (2015-04-04)
Immunoglobulin E (IgE) plays a central role in type I hypersensitivity including allergy and asthma. Novel treatment strategy envisages development of a therapeutic vaccine designed to elicit autologous blocking antibodies against the IgE. We sought to develop an IgE-epitope antigen
Liqin Wang et al.
Journal of biomedicine & biotechnology, 2012, 161372-161372 (2012-06-08)
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can
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