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Merck
CN

B5525

Sigma-Aldrich

溴酚蓝 钠盐

别名:

3′,3′′,5′,5′′-四溴酚磺酞 钠盐

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About This Item

经验公式(希尔记法):
C19H9Br4NaO5S
CAS号:
分子量:
691.94
Beilstein:
6995701
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.52

形式

powder

技术

electrophoresis: suitable

溶解性

water: soluble

适用性

suitable for nucleic acid electrophoresis

储存温度

room temp

SMILES字符串

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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一般描述

溴酚蓝是一种pH指示剂,并且是一种强蓝色染料。 它常被用作琼脂糖或聚丙烯酰胺凝胶电泳的示踪染料。 溴酚蓝具有微弱的负电荷并且与DNA同向迁移,使用户能够监测分子在凝胶中迁移的进程。 不同的凝胶组成具有不同的迁移速率。

应用

溴酚蓝钠盐已用于制备蛋白质样品,以便进行蛋白质印迹分析。它也被添加到果蝇的食物中,辅助肠道显色,便于解剖。
用于琼脂糖或聚丙烯酰胺凝胶中核酸或蛋白质电泳的示踪染料。

重悬

使用含0.015%溴酚蓝的标准配方,可制备6x凝胶上样缓冲液。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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