B6650
X-GlcA
reagent for selection of recombinant bacterial clones
别名:
5-溴-4-氯-3-吲哚基 β-D-葡糖醛酸 环己铵盐, X-GlcA, X-葡糖苷酸 CHA 盐
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关于此项目
经验公式（希尔记法）:
C14H13BrClNO7 · C6H13N
化学文摘社编号:
分子量:
521.79
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.85
等级
Molecular Biology
无菌性
non-sterile
方案
≥98% (TLC)
表单
powder
技术
nucleic acid detection: suitable
溶解性
DMF: soluble
适用性
suitable for β-galactosidase test
储存温度
−20°C
SMILES字符串
NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34
InChI
1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1
InChI key
JXCKZXHCJOVIAV-CYRSAHDMSA-N
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相关类别
一般描述
X-GlcA 是 β-半乳糖苷酶的显色底物，用于确定琼脂平板上生长的细菌中是否存在克隆的 DNA 插入片段。 X-GlcA 设计用于具有 lac+ 表型的重组细菌菌落的蓝白色选择中替代 X-Gal。
应用
β-葡萄糖醛酸酶 (GUS) 基因检测的显色底物。
适用于选择具有 lac+ 表型的重组细菌菌落。
X-GlcA 已用于根切片的组化染色，用于光学显微镜观察。
X-GlcA 已用于根切片的组化染色，用于光学显微镜观察。
生化/生理作用
当 X-GlcA 被 β-半乳糖苷酶水解时，所得产物将形成蓝色沉淀。在 X-GlcA 存在下生长的 Lac+ 菌落会变成深蓝色，从而易于区分 lac+ 和 lac- 菌落。
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