B7542
马来酰亚胺活化 BSA
别名:
Maleimide Activated Bovine Serum Albumin
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352111
Biological source:
bovine
Form:
lyophilized powder
Technique(s):
ELISA: suitable, bioconjugation: suitable
Concentration:
5—6 mg/mL protein
biological source
bovine
sterility
non-sterile
form
lyophilized powder
concentration
5—6 mg/mL protein
technique(s)
ELISA: suitable, bioconjugation: suitable
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
bovine ... ALB(280717)
General description
马来酰亚胺活化 BSA 以冻干、即用型形式提供，无需耗时的载体蛋白活化和纯化步骤。对于每个 KLH 分子，马来酰亚胺活化的 BSA 含有大约 5-15 个马来酰亚胺基团。在 pH 6.6-7.0 条件下进行与含半胱氨酸肽的偶联反应。然后可通过 Ellman 试剂比色法测定偶联程度。用于检测含半胱氨酸肽中未反应的巯基。
Application
马来酰亚胺活化 BSA (mBSA) 的表面具有活性马来酰亚胺基团，可与含半胱氨酸的肽或含巯基的半抗原偶联。马来酰亚胺基团和巯基的反应在温和的偶联条件 (pH 6.5-7.5) 下快速选择性地进行，得到稳定的、共价连接的肽-蛋白偶联物，可用于免疫方案或抗体筛选方法。
马来酰亚胺活化的 BSA 已用于肽偶联。
Physical form
每瓶含 5 mg 从 10 mM 磷酸钠缓冲液 (pH 6.6) 冻干的马来酰亚胺活化 BSA，以 0.115 mM NaCl、1 mM EDTA 和 40 mM 蔗糖作为稳定剂。
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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