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Merck
CN

B7554

抗-c-Myc−生物素抗体,小鼠单克隆

clone 9E10, purified from hybridoma cell culture

别名:

抗-c-Myc, 单克隆抗 -c-Myc−生物素 小鼠抗

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关于此项目

NACRES:
NA.56
UNSPSC Code:
12352203
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产品名称

抗-c-Myc−生物素抗体,小鼠单克隆, clone 9E10, purified from hybridoma cell culture

biological source

mouse

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

9E10, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

0.8 mg/mL

technique(s)

western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... MYC(4609)

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Application

成功使用该抗体的应用以及相关的同行评审论文如下。
酶联免疫吸附试验(1篇)
该抗体适用于免疫印迹。

Biochem/physiol Actions

c-Myc肽(产品代码M2435)特异性抑制了免疫印迹染色。

General description

单克隆抗c-Myc小鼠抗体分离自腹水,并与生物素偶联。 当在融合蛋白的N末端或C末端表达时,该抗体可识别c-Myc标记的融合蛋白上的c-Myc标签序列。

Immunogen

人p62c-Myc蛋白的合成肽。

Physical form

溶于含有1%牛血清白蛋白和15mM叠氮化钠(作为防腐剂)的0.01M磷酸盐缓冲盐水(pH7.4)中。

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存储类别

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Illari Salvatori et al.
Journal of neurochemistry, 146(5), 585-597 (2018-05-21)
TAR DNA-binding protein 43 (TDP-43) is an RNA-binding protein and a major component of protein aggregates found in amyotrophic lateral sclerosis and several other neurodegenerative diseases. TDP-43 exists as a full-length protein and as two shorter forms of 25 and
Steven R Bates et al.
Applied physics letters, 95(7), 73705-73705 (2009-09-25)
We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association
Yan Wang et al.
PloS one, 7(12), e52404-e52404 (2012-12-29)
The neural cell adhesion molecule L1 plays important roles in neuronal migration and survival, neuritogenesis and synaptogenesis. L1 has also been found in tumors of different origins, with levels of L1 expression correlating positively with the metastatic potential of tumors.
Ilja Demuth et al.
Oncogene, 23(53), 8611-8618 (2004-10-07)
DNA interstrand crosslinks (ICLs) are critical lesions for the mammalian cell since they affect both DNA strands and block transcription and replication. The repair of ICLs in the mammalian cell involves components of different repair pathways such as nucleotide-excision repair
Romain Rouet et al.
Nature protocols, 7(2), 364-373 (2012-02-04)
Here we describe protocols for the expression of human antibody fragments in Escherichia coli. Antigen-specific clones are identified by soluble fragment ELISA and concentrated by periplasmic preparation. They are then further purified by affinity chromatography. This article provides an overview

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