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Merck
CN

B8438

阿尔新蓝 溶液

1% in 3% acetic acid, pH 2.5

别名:

阿尔新蓝8XG 溶液

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关于此项目

NACRES:
NA.47
UNSPSC Code:
12171500
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产品名称

阿尔新蓝 溶液, 1% in 3% acetic acid, pH 2.5

form

liquid

concentration

1% in 3% acetic acid, pH 2.5

technique(s)

microbe id | staining: suitable

color

dark blue to very dark blue

pH range

2.4-2.6

Amax

0.45-0.60 (1:400 in H2O)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

Application

阿尔新蓝溶液已用于对羧化或硫酸化的酸性糖胺聚糖(GAG;粘多糖)进行组织学染色。在pH为2.5时,硫酸糖胺聚糖似乎是阿尔新蓝的首选底物。阿尔新蓝用于染色透明质酸(HA,一种非硫酸化糖胺聚糖),但透明质酸的染色强度受所用组织固定方法的影响。组织中糖胺聚糖的阿尔新蓝染色,可以通过添加诸如氯化镁的电解质来改进。

Biochem/physiol Actions

阿尔新蓝是一种阳离子染料。它与酸性糖胺聚糖形成不溶性复合物,从而有助于糖胺聚糖的定量测定。它还用于骨和软骨的染色。

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.
Wassersug R J.
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SPECIFIC STAINING OF SULPHATE GROUPS WITH ALCIAN BLUE AT LOW PH.
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Mesenchymal stem-cell based therapies have been proposed as novel treatments for intervertebral disc (IVD) degeneration. The development of these treatment strategies, however, has been hindered by the incomplete understanding of the origin, biological properties of nucleus pulposus (NP) derived stem/progenitor
W Lin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
Whiteman P.
The Biochemical Journal, 131(2), 351-357 (1973)

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