产品名称
抗- 钙蛋白单克隆抗体 小鼠抗, clone hCP, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
hCP, monoclonal
mol wt
antigen 34 kDa
contains
15 mM sodium azide
species reactivity
rat, rabbit, pig, human, mouse
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:10,000 using human uterus extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CNN1(1264), CNN2(1265)
mouse ... Cnn1(12797), Cnn2(12798)
rat ... Cnn1(65204), Kcnip4(259243)
Application
小鼠产生的抗-钙调蛋白单克隆抗体适用于以下应用：
- 使用福尔马林固定、石蜡包埋切片的免疫组化
- 免疫沉淀
- 微阵列
- 蛋白质印迹（使用人子宫提取物，稀释度为 1:10,000）
- 流式细胞仪分析
Biochem/physiol Actions
hCP抗体（又称CALP）可定位哺乳动物平滑肌中的钙调蛋白。在人子宫中，一种约 27 kDa 的附加条带(1-钙调蛋白)也可能随之染色。该抗体不会与骨骼肌、心脏或非肌肉组织的钙调蛋白发生交叉反应。然而，该抗体与人或小鼠骨骼肌中的表位(150 kDa 范围)表现出交叉反应。在免疫组化染色中，该产品表现出平滑肌特异性。它可以染色组织切片和原代培养细胞(或早期传代细胞)中的血管和内脏平滑肌细胞，但不能染色大多数最初源自平滑肌的细胞系。该抗体不能染色上皮、内皮或结缔组织成纤维细胞。 该产品不与鸡肉的平滑肌组织发生交叉反应。
钙调蛋白是钙结合蛋白，属于肌动蛋白相关蛋白家族。钙调蛋白是血管平滑肌(VSM)中蛋白激酶 C(PKC)自磷酸化所必需的。钙调蛋白-1 抑制可防止子宫平滑肌细胞迁移、引起形态变化和F-肌动蛋白重排，而不会影响其增殖和凋亡。钙调蛋白 H1(CN)是平滑肌细胞的分化标志物，在几种人类肿瘤的血管中下调。它可以抑制肌动球蛋白 ATP 酶活性。h1 和 h2 钙调蛋白与 F-肌动蛋白结合，并在调节平滑肌和非肌肉细胞的肌动蛋白丝中发挥关键作用。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
人子宫平滑肌提取物。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源生物产品
此项目有
M G Frid et al.
Developmental biology, 153(2), 185-193 (1992-10-01)
Expression of the regulatory contractile proteins, heavy caldesmon (h-caldesmon) and calponin was studied in human aortic smooth muscle cells (SMCs) during development and compared with the expression of alpha-SM-actin and smooth muscle-myosin heavy chain (SM-MHCs). For this study, novel monoclonal
Won Sun Park et al.
American journal of physiology. Cell physiology, 305(4), C377-C391 (2013-06-14)
Human adipose tissue-derived mesenchymal stem cells (hASCs) have the power to differentiate into various cell types including chondrocytes, osteocytes, adipocytes, neurons, cardiomyocytes, and smooth muscle cells. We characterized the functional expression of ion channels after transforming growth factor-β1 (TGF-β1)-induced differentiation
A Draeger et al.
FEBS letters, 291(1), 24-28 (1991-10-07)
Two-dimensional gel analysis of basic proteins in developing human smooth muscle identifies calponin as a prominent marker of the differentiated phenotype. Adult tissue (human and mouse) typically expresses up to four calponin isoforms, three of which appear sequentially during fetal
Jie Liu et al.
American journal of physiology. Lung cellular and molecular physiology, 300(5), L710-L717 (2011-03-01)
Perinatal exposure to maternal smoke is associated with adverse pulmonary effects, including reduced lung function and increased incidence of asthma. However, the mechanisms underlying these effects are unknown, and there is no effective preventive and/or therapeutic intervention. Recently, we suggested
Y Yanagisawa et al.
European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 34(5), 531-537 (2007-08-21)
Calponin h1 (CN) is a differentiation marker of smooth muscle cells that has been reported to be down-regulated in the blood vessels of several human tumors. In this study, we examined CN expression in blood vessels in relation to the
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