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C3142

Sigma-Aldrich

α-糜蛋白酶 来源于牛胰腺

(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

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别名:
胰凝乳蛋白酶
CAS号:
9004-07-3
EC 号:
3.4.21.1 (BRENDA, IUBMB)
EC 号:
232-671-2
MDL编号:
MFCD00130481
eCl@ss:
42010112
NACRES:
NA.54

类型

Type VII

形式

essentially salt-free, lyophilized powder

specific activity

≥40 units/mg protein

分子量

25 kDa

溶解性

1 mM HCl: soluble 10 mg/mL, clear

UniProt登记号

P00767

储存温度

−20°C

Gene Information

cow ... CTRB1(618826)

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相关类别

应用

用 Sigma 公司的 α-糜蛋白酶与蝗虫游走蛋白 (Locusta migratoria) 形成复合物，测定了两个同源抑制剂（大脑间部主肽-C 和大脑间部主肽-D2v）的晶体结构。

生化/生理作用

α-糜蛋白酶是一种丝氨酸肽酶，在由二硫键连接的三条多肽链（a 链-13 残基、B 链-131 残基和 C 链-97 残基）中含有 241 个氨基酸残基。发现该酶的分子量为 25 kDa。其 pI 为 8.75。选择性水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸 C 末端的肽键。ca2+ 激活并稳定酶。该酶被二异丙基氟磷酸盐 (DFP)、苯甲基磺酰氟 (PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮 (TPCK)、糜蛋白酶抑制剂、抑肽酶、α1-抗胰蛋白酶、α2-巨球蛋白、10 mM cu2 + 和 hg2 + 抑制。
一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。

单位定义

在 pH 值为 7.8、25°C 条件下，1 个单位每分钟水解 1.0 μmol BTEE。

制备说明

TLCK处理灭活可能存在于胰凝乳蛋白酶中的胰蛋白酶，而不影响胰凝乳蛋白酶活性。

分析说明

通过 A1%/280 测定蛋白

其他说明

更多关于糜蛋白酶的信息请见www.sigma-aldrich.com/enzymeexplorer

底物

产品编号
说明
价格

抑制剂

产品编号
说明
价格

A2504

6-氨基己酸, ≥99% (titration), powder

A7824

6-氨基己酸, BioUltra, ≥99%

D0879

氟磷酸二异丙酯

L2023

亮肽素 三氟乙酸盐, ≥90% (HPLC), microbial

T9777

胰蛋白酶-胰凝乳蛋白酶抑制剂 来源于大豆, lyophilized powder

A1153

抑肽酶 来源于牛肺, lyophilized powder, 3-8 TIU/mg solid

P7626

苯甲磺酰氟, ≥98.5% (GC)

B1552

溴烯醇内酯, ≥98% (TLC)

A9024

α1-抗胰蛋白酶 来源于人类血浆, salt-free, lyophilized powder

A6150

α1-抗胰蛋白酶 来源于人类血浆, salt-free, lyophilized powder

A9285

α1-Antichymotrypsin 来源于人类血浆, ≥92.5% (SDS-PAGE), lyophilized powder

T4376

N-对-甲苯磺酰基-L-苯基乙基氯甲基酮, ≥97% (TLC), powder

C7268

胰凝乳蛋白酶抑制剂, microbial

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of alpha-chymotrypsin from bovine pancreas (Product No. C3142)?

    The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.This information and the basic structure description of the enzyme can be found on our product information sheet (under Documents, above) at our website.

  6. How do you recommend preparing a solution of Product No. C3142?

    It is recommended to reconstitute Product No. C3142 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as both a stabilizer (and possibly an activator) of the enzyme.

  7. How can solutions of alpha-chymotrypsin (Product No. C3142) be stored?

    Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.

  8. What is the pH optimum for the enzyme, Product No. C3142?

    The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

A Roussel et al.
The Journal of biological chemistry, 276(42), 38893-38898 (2001-08-10)
The crystal structures of two homologous inhibitors (PMP-C and PMP-D2v) from the insect Locusta migratoria have been determined in complex with bovine alpha-chymotrypsin at 2.1- and 3.0-A resolution, respectively. PMP-C is a potent bovine alpha-chymotrypsin inhibitor whereas native PMP-D2 is
Tobias Koschubs et al.
Nucleic acids research, 38(10), 3186-3195 (2010-02-04)
Mediator is the central coactivator complex required for regulated transcription by RNA polymerase (Pol) II. Mediator consists of 25 subunits arranged in the head, middle, tail and kinase modules. Structural and functional studies of Mediator are limited by the availability
Dhwani S Korde et al.
MethodsX, 9, 101779-101779 (2022-07-23)
Alzheimer´s disease is characterized by hyperphosphorylated tau neurofibrillary tangles and beta-amyloid plaques. Both molecules can be easily measured in human fluids or tissue extracts by immunoassays. However, the different molecular weight species can only be differentiated on Western Blot gels.
Tommi Kotila et al.
Nature communications, 13(1), 3442-3442 (2022-06-16)
Actin polymerization generates forces for cellular processes throughout the eukaryotic kingdom, but our understanding of the 'ancient' actin turnover machineries is limited. We show that, despite > 1 billion years of evolution, pathogenic Leishmania major parasite and mammalian actins share the same
M Gestin et al.
Digestive diseases and sciences, 42(6), 1302-1311 (1997-06-01)
A specific method for pancreatic elastase II activity analysis was developed. True elastase II activity could be discriminated from that of elastase I and chymotrypsin. The postnatal development of four pancreatic proteases in the duodenal juice of children and in
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商品

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

实验方案

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

α-胰凝乳蛋白酶的酶学测定程序（EC 3.4.21.1）

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

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