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C3142

α-糜蛋白酶来源于牛胰腺

Name: α-糜蛋白酶 来源于牛胰腺
Brand: SIGMA

(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

别名:

胰凝乳蛋白酶

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关于此项目

化学文摘社编号:

9004-07-3

UNSPSC Code:

12352204

eCl@ss:

42010112

EC Number:

232-671-2

NACRES:

NA.54

MDL number:

MFCD00130481

EC 号:

3.4.21.1 (BRENDA, IUBMB)

Specific activity:

≥40 units/mg protein

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Quality Level

200

type

Type VII

form

essentially salt-free, lyophilized powder

specific activity

≥40 units/mg protein

mol wt

25 kDa

solubility

1 mM HCl: soluble 10 mg/mL, clear

UniProt accession no.

P00767

storage temp.

−20°C

Gene Information

cow ... CTRB1(618826)

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Application

用 Sigma 公司的 α-糜蛋白酶与蝗虫游走蛋白 (Locusta migratoria) 形成复合物，测定了两个同源抑制剂（大脑间部主肽-C 和大脑间部主肽-D2v）的晶体结构。

Biochem/physiol Actions

α-糜蛋白酶是一种丝氨酸肽酶，在由二硫键连接的三条多肽链（a 链-13 残基、B 链-131 残基和 C 链-97 残基）中含有 241 个氨基酸残基。发现该酶的分子量为 25 kDa。其 pI 为 8.75。选择性水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸 C 末端的肽键。ca²⁺ 激活并稳定酶。该酶被二异丙基氟磷酸盐 (DFP)、苯甲基磺酰氟 (PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮 (TPCK)、糜蛋白酶抑制剂、抑肽酶、α₁-抗胰蛋白酶、α₂-巨球蛋白、10 mM cu² + 和 hg² + 抑制。

一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。

Preparation Note

TLCK处理灭活可能存在于胰凝乳蛋白酶中的胰蛋白酶，而不影响胰凝乳蛋白酶活性。

Analysis Note

通过 A1%/280 测定蛋白

Other Notes

在 pH 值为 7.8、25°C 条件下，1 个单位每分钟水解 1.0 μmol BTEE。

更多关于糜蛋白酶的信息请见www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08,GHS07,GHS09

signalword

Danger

hcodes

H302,H315,H319,H334,H335,H400

pcodes

P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

动植物源性产品

低风险生物材料

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商品

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

实验方案

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

α-胰凝乳蛋白酶的酶学测定程序（EC 3.4.21.1）

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

全球贸易项目编号

货号	GTIN
C3142-25MG	04061833485460
C3142-100MG	04061833485446
C3142-10MG	04061833485453

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