产品名称
羧肽酶 Y 来源于面包酵母(酿酒酵母), lyophilized powder, ≥50 units/mg protein
grade
Proteomics Grade
form
lyophilized powder
specific activity
≥50 units/mg protein
mol wt
61 kDa
composition
Protein, ≥75% E1%/280
shipped in
wet ice
storage temp.
−20°C
Quality Level
正在寻找类似产品? 访问 产品对比指南
Analysis Note
可能存在酰胺酶和酯酶活性
Application
羧肽酶Y具有广泛的特异性并且在尿素中稳定。因此,该酶与其他羧肽酶不同,可以用于序列分析。由于其酰胺酶作用,该酶可用于具有酰胺化COOH-末端基团的肽,例如催产素和加压素的序列分析。
Biochem/physiol Actions
糖蛋白的分子量约为61,000,氮含量为12.74%。它是一个含有442个残基的单条多肽链,在碳水化合物部分含有16个葡糖胺残基。赖氨酸位于NH2末端,-Asp-Ser-Thr-Leu位COOH末端序列。该酶的主要作用是从多肽链上去除COOH末端残基。它可用作液泡标记酶,用于蛋白质转运和定位研究。
Other Notes
基于EM/230 = 191.5,在25°C,pH 6.75的条件下,一个单位每分钟可将1.0 mole μN-CBZ-Phe-Ala水解为N-CBZ-L-苯丙氨酸和L-丙氨酸。
Packaging
包装尺寸基于蛋白质含量
Physical form
含有柠檬酸的冻干粉末。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Caroline A Magalhães et al.
Memorias do Instituto Oswaldo Cruz, 106(2), 146-152 (2011-05-04)
Typical and atypical enteropathogenic Escherichia coli (EPEC) are considered important bacterial causes of diarrhoea. Considering the repertoire of virulence genes, atypical EPEC (aEPEC) is a heterogeneous group, harbouring genes that are found in other diarrheagenic E. coli pathotypes, such as
Johanna Rankenberg et al.
Experimental eye research, 210, 108704-108704 (2021-07-25)
Advanced glycation end products (AGEs) accumulate with age in human lens capsules. AGEs in lens capsules potentiate the transforming growth factor beta-2-mediated mesenchymal transition of lens epithelial cells, which suggests that they play a role in posterior capsule opacification after
H R Stennicke et al.
Biochemistry, 35(22), 7131-7141 (1996-06-04)
The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as
E van Tuinen et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35(3), 327-333 (1987-03-01)
We have developed a simple and effective method to embed whole yeast cells in Lowicryl resins with excellent ultrastructural and antigenic preservation. Using affinity-purified antibodies eluted from electrophoretically separated proteins transferred to nitrocellulose, we have shown by immunoelectron microscopy that
Keisuke Obara et al.
Journal of lipid research, 54(3), 831-842 (2013-01-18)
Very long-chain fatty acids (VLCFAs), fatty acids with chain-length greater than 20 carbons, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In the present study, we screened for multicopy suppressors that
相关内容
QC Methods
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持
