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C4129

α-糜蛋白酶来源于牛胰腺

Name: α-糜蛋白酶 来源于牛胰腺
Brand: SIGMA

Type II, lyophilized powder, ≥40 units/mg protein

别名:

α-chymotrypsin A and B, alpha-chymotrypsin

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关于此项目

化学文摘社编号:

9004-07-3

UNSPSC Code:

12352204

eCl@ss:

42010112

EC Number:

232-671-2

NACRES:

NA.54

MDL number:

MFCD00130481

EC 号:

3.4.21.1 (BRENDA, IUBMB)

Specific activity:

≥40 units/mg protein

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type

Type II

Quality Level

300

form

lyophilized powder

specific activity

≥40 units/mg protein

mol wt

25 kDa

composition

protein, ≥85%

color

white to off-white

UniProt accession no.

P00767

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

cow ... CTRB1(618826)

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Application

sigma 公司的酶已被用于研究糖基化 α-胰凝乳蛋白酶的结构 - 功能关系，使用固定化金属离子亲和层析（IMAC）和固定化金属离子亲和毛细管电泳（IMACE）。

Biochem/physiol Actions

α-胰凝乳蛋白酶是丝氨酸肽酶，并且具有通过二硫键连接的三条多肽链（A 链-13 残基，B 链-131 残基和 C 链-97 残基）中包含的 241 个氨基酸残基。发现该酶的分子量为 25kDa。pI 是 8.75。它选择性地水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸的 C-末端侧的肽键。Ca²⁺ 激活和稳定酶。该酶被二异丙基氟磷酸盐（DFP）、甲苯磺酰氟（PMSF）、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮（TPCK）、胰凝乳蛋白酶抑制剂、抑肽酶、α1-抗胰蛋白酶和 α2-巨球蛋白、10mM Cu²⁺ 和 Hg²⁺ 抑制。

一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。

Preparation Note

以 3×结晶的胰凝乳蛋白酶原为原料生产

Analysis Note

通过 E1％/280 确定的蛋白质

Other Notes

一个单元将在 pH 7.8，25℃下，每分钟水解 1.0 μmole BTEE。

有关胰凝乳蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08,GHS07,GHS09

signalword

Danger

hcodes

H302,H315,H319,H334,H335,H400

pcodes

P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

低风险生物材料

动植物源性产品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000529055

0000532704

0000529056

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商品

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

实验方案

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

α-胰凝乳蛋白酶的酶学测定程序（EC 3.4.21.1）

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

全球贸易项目编号

货号	GTIN
C4129-1G	04061833491683
C4129-250MG	04061833491690
C4129-10G	04061833491676
C4129-500MG	04061833491706

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