产品名称
单克隆抗肌动蛋白抗体, clone AC-40, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC-40, monoclonal
form
buffered aqueous solution
mol wt
antigen 42 kDa
species reactivity
human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat
technique(s)
direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
已使用肌动蛋白单克隆抗体作为一抗，对MDCK细胞裂解物进行蛋白质免疫印迹分析。
肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析，以确保等量蛋白质上样。
Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining.
General description
该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle).
Immunogen
与多抗原肽（MAP）骨架连接的合成肌动蛋白C末端肽
Biochem/physiol Actions
Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Legal Information
Distributed under license from Amersham Biosciences Limited.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
Gunning P, et al.
Molecular and Cellular Biology, 3(5), 787-795 (1983)
Erin Faught et al.
Scientific reports, 8(1), 18081-18081 (2018-12-29)
The mineralocorticoid receptor (MR) in mammals mediates the effects of aldosterone in regulating fluid balance and potassium homeostasis. While MR signalling is essential for survival in mammals, there is no evidence that MR has any physiological role in ray-finned fish.
Xiao-Yan Wen et al.
JCI insight, 3(24) (2018-12-21)
Sialic acids are important components of glycoproteins and glycolipids essential for cellular communication, infection, and metastasis. The importance of sialic acid biosynthesis in human physiology is well illustrated by the severe metabolic disorders in this pathway. However, the biological role
A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
Skalli O, et al.
The Journal of Cell Biology, 103(6), 2787-2796 (1986)
Wentao Shi et al.
Molecular medicine reports, 20(3), 2763-2773 (2019-07-20)
Ectomesenchymal stem cells (EMSCs) represent a type of adult stem cells derived from the cranial neural crest. These cells are capable of self‑renewal and have the potential for multidirectional differentiation. Tissue transglutaminase type 2 (TG2) is a ubiquitously expressed member
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