biological source
mouse
Quality Segment
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1A4, monoclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa
species reactivity
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
storage condition
protect from light
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix
isotype
IgG2a
UniProt accession no.
application(s)
research pathology
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
General description
其抗体(也称为抗α-Sm-1)对α-平滑肌肌动蛋白的单一异构体具有特异性。它在免疫印迹试验中可与α-平滑肌肌动蛋白发生特异性反应,并可在冷冻或福尔马林固定的、或石蜡包埋的组织切片中对平滑肌细胞进行标记。
单克隆抗肌动蛋白α-平滑肌(小鼠IgG2a同种型)源自小鼠骨髓瘤细胞和免疫BALB/c小鼠脾细胞融合产生的1A4杂交瘤
Immunogen
N-末端合成的α-平滑肌肌动蛋白十肽。
Application
使用单克隆抗ACTA2抗体对来自牛主动脉的平滑肌细胞进行免疫细胞化学分析。首先将细胞在玻璃盖玻片上生长,并在4%的50%丙酮/乙醇中固定10分钟。
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Biochem/physiol Actions
在鸡和大鼠胚胎成肌过程中,平滑肌肌动蛋白可与肌节α-肌动蛋白进行瞬时共表达。在成年哺乳动物心脏的心室传导通道中,经证存在这种物质。它在平滑肌瘤、平滑肌肉瘤和平滑肌母细胞瘤以及部分横纹肌肉瘤中表达。
Physical form
溶解保存在0.01M磷酸缓冲盐,pH7.4, 含有1%牛血清白蛋白和15 mM叠氮化钠。
Other Notes
想要查看肌动蛋白抗体选择指南,请访问 www.sigmaaldrich.com/actin。
Legal Information
Cy在 Amersham Biosciences Limited许可下销售。
Cy3 is a trademark of Cytiva
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable