C6423
α-Chymotrysin Protease
Cleaves at aromatic residues, suitable for mass spectrometry, from bovine pancreas
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
42010112
NACRES:
NA.26
EC Number:
232-671-2
MDL number:
产品名称
α-糜蛋白酶 来源于牛胰腺, suitable for protein sequencing, salt-free, lyophilized powder
grade
Proteomics Grade
Quality Level
form
salt-free, lyophilized powder
mol wt
25 kDa
suitability
suitable for protein sequencing
UniProt accession no.
storage temp.
2-8°C
Gene Information
cow ... CTRB1(618826)
General description
胰凝乳蛋白酶(Chy)是一种丝氨酸蛋白酶。它的分子量为25.7kDa，广泛应用于医药行业。它由胰凝乳蛋白酶原合成，这种转化过程需要钙。
牛胰 α-糜蛋白酶 (BPC) 可催化 Povarov 反应制备四氢喹啉衍生物。牛胰 α-糜蛋白酶 (CHT) 是一种酶蛋白。已报道乙醇、1,4-二恶烷和乙腈等不同浓度的有机溶剂对 CHT 的影响。
Application
牛胰 α-糜蛋白酶有以下用途：
- 通过质谱法 (MS) 鉴定蛋白质
- 肌球蛋白重链的分离与鉴定
- 毒素制剂
- 用于 SDS-PAGE 分析细胞蛋白的感染和未感染细胞孵育
Biochem/physiol Actions
α-牛胰腺胰凝乳蛋白酶通过Ca2+实现稳定并且催化肽键的水解，尤其氨基酸酪氨酸、苯基丙氨酸、色氨酸以及C端亮氨酸。α-胰凝乳蛋白酶受Cu2+和Hg的抑制。
一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。
Other Notes
在 pH 7.8、25°C 条件下，1 个单位将每分钟水解 1.0 μmol BTEE。
查看更多信息 糜蛋白酶 的信息，请访问 www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
低风险生物材料
此项目有
商品
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.
The α-chymotrypsin-catalyzed Povarov reaction: one-pot synthesis of tetrahydroquinoline derivatives.
Li LP, et al.
Green Chemistry, 17(5), 3148-3156 (2015)
Miguel Ribeiro et al.
International journal of molecular sciences, 14(3), 5650-5667 (2013-03-13)
Analysis of Portuguese wheat (Triticum aestivum L.) landrace 'Barbela' revealed the existence of a new x-type high molecular weight-glutenin subunit (HMW-GS) encoded at the Glu-A1 locus, which we named 1Ax1.1. Using one-dimensional and two-dimensional electrophoresis and mass spectrometry, we compared
Xinyu Liu et al.
eLife, 9 (2020-12-05)
The production of reactive oxygen species (ROS) is a ubiquitous defense response in plants. Adapted pathogens evolved mechanisms to counteract the deleterious effects of host-derived ROS and promote infection. How plant pathogens regulate this elaborate response against ROS burst remains
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C6423-25UG | 04061833511190 |
| C6423-.1MG | 04061832855981 |