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C7762

α-糜蛋白酶来源于牛胰腺

Name: α-糜蛋白酶 来源于牛胰腺
Brand: SIGMA

Type I-S, essentially salt-free, lyophilized powder

别名:

α-chymotrypsin A and B, alpha-chymotrypsin

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关于此项目

化学文摘社编号:

9004-07-3

UNSPSC Code:

12352204

eCl@ss:

42010112

EC Number:

232-671-2

NACRES:

NA.54

MDL number:

MFCD00130481

EC 号:

3.4.21.1 (BRENDA, IUBMB)

Specific activity:

≥40 units/mg protein

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type

Type I-S

Quality Level

200

form

essentially salt-free, lyophilized powder

specific activity

≥40 units/mg protein

mol wt

25 kDa

purified by

3× crystallization

solubility

1 mM HCl: soluble 2.0 mg/mL, clear

UniProt accession no.

P00767

storage temp.

−20°C

Gene Information

cow ... CTRB1(618826)

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Application

该产品已被用于研究几种醚低聚物对酶的抑制作用。它也被用于裂解酚氧化酶前体，顺序估算血淋巴中的总酚氧化酶。此外，在使用 Deraeocoris nigritulus 的唾液腺和前中肠提取物的糜蛋白酶试验中，将该酶用作阳性对照。

在一项研究中使用了来自牛胰腺的 α-糜蛋白酶，以研究通过 Winsor-III 微乳液系统提取蛋白质。来自牛胰腺的 α-胰凝乳蛋白酶也被用于研究胰蛋白酶的一种新的基于富勒烯的特异性荧光探针。

Biochem/physiol Actions

α-糜蛋白酶是一种丝氨酸肽酶，在由二硫键连接的三条多肽链（a 链-13 残基、B 链-131 残基和 C 链-97 残基）中含有 241 个氨基酸残基。发现该酶的分子量为 25 kDa。其 pI 为 8.75。选择性水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸 C 末端的肽键。ca²⁺ 激活并稳定酶。该酶被二异丙基氟磷酸盐 (DFP)、苯甲基磺酰氟 (PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮 (TPCK)、糜蛋白酶抑制剂、抑肽酶、α10-抗胰蛋白酶、α2-巨球蛋白、10 mM cu² + 和 hg² + 抑制。

一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。

Preparation Note

制备无自溶产物和低分子量污染物。

可在 1 mM HCl 中复溶 2 mg/mL 浓度的粉末，以形成澄清溶液。

Analysis Note

E1%/280法测定蛋白

最低 85% 蛋白质

Other Notes

在 pH 7.8、25°C 条件下，1 个单位将每分钟水解 1.0 μmol BTEE。

有关胰凝乳蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08,GHS07,GHS09

signalword

Danger

hcodes

H302,H315,H319,H334,H335,H400

pcodes

P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

低风险生物材料

动植物源性产品

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Synthesis of ether oligomers.

Olivier Renaudet et al.

Organic letters, 6(3), 397-400 (2004-01-30)

[structure: see text] Hydroxyaromatic aldehydes and ketones were used as building blocks to prepare ether oligomers. An iterative two-step protocol involving Mitsunobu coupling and carbonyl reduction provided a protecting-group-free route with high yields. Activity screening of an 84-member library against

Exposure to sexual signals during rearing increases immune defence in adult field crickets.

Nathan W Bailey et al.

Biology letters, 7(2), 217-220 (2010-09-03)

Increased investment in immunity is expected to be beneficial under crowded conditions because of the greater risk of pathogen and parasite transmission, but the evolution of this facultative response relies on the ability to accurately assess social cues in the

Protein-coated polymer as a matrix for enzyme immobilization: immobilization of trypsin on bovine serum albumin-coated allyl glycidyl ether-ethylene glycol dimethacrylate copolymer.

Lakshmi Swarnalatha Jasti et al.

Biotechnology progress, 30(2), 317-323 (2014-01-23)

Allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer with 25% crosslink density (AGE-25) shows excellent bovine serum albumin (BSA) adsorption (up to 16% (w/w)) at pH 8.0 and the adsorbed BSA is strongly bound. This protein-coated polymer provides a novel

Digestive enzymes and stylet morphology of Deraeocoris nigritulus (Uhler)(Hemiptera: Miridae) reflect adaptations for predatory habits.

Boyd Jr, D. W.

Annals of the Entomological Society of America, 96(5), 667-671 (2003)

Discrimination by heat and proteinase treatments between flocculent phenotypes conferred on Saccharomyces cerevisiae by the genes FLO1 and FLO5.

J A Hodgson et al.

Journal of general microbiology, 131(12), 3219-3227 (1985-12-01)

The effects of elevated temperature and of digestion with a variety of proteinases on the flocforming ability of flocculent strains of Saccharomyces cerevisiae, both genetically defined (FLO1 and FLO5) laboratory and genetically undefined brewing strains, have been determined. This has

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商品

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

实验方案

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

α-胰凝乳蛋白酶的酶学测定程序（EC 3.4.21.1）

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

全球贸易项目编号

货号	GTIN
C7762-100MG	04061833521083
C7762-5MG	04061833521106
C7762-25MG	04061833521090

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