C7855
抗-Cdh1抗体，小鼠单克隆抗体
clone DCS-266, purified from hybridoma cell culture
登录 查看组织和合同定价。
选择尺寸
关于此项目
Conjugate:
unconjugated
Clone:
DCS-266, monoclonal
Application:
ARR, IP, WB
Citations:
7
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
DCS-266, monoclonal
form
buffered aqueous solution
mol wt
antigen 50 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
immunoprecipitation (IP): suitable, microarray: suitable, western blot: 2-4 μg/mL using a HeLa cell nuclear extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CDH1(999)
相关类别
General description
上皮细胞中的Cdh1同源主要细胞粘附分子属于钙黏着蛋白超家族。
单克隆抗-Cdh1（小鼠IgG1亚型）来自DCS-266杂交瘤，其通过融合小鼠骨髓瘤细胞和用重组人钙黏着蛋白-1（Cdh1）免疫的BALB/c小鼠的脾细胞产生。
Immunogen
重组人Cdh1。
Application
在鉴定新型人类Cdt1结合蛋白的研究中，单克隆抗Cdh1抗体用于裂解物的免疫印迹。通过使用HeLa细胞核提取物，适用于2-4 μg/mL的蛋白质印迹。也适用于免疫沉淀和微阵列。
Biochem/physiol Actions
单克隆抗Cdh1与人Cdh1特异性反应。
单克隆抗Cdh1与人Cdh1特异性反应。在真核生物中，细胞周期进程的调节取决于细胞周期蛋白的表达。有丝分裂细胞周期蛋白的降解遵循泛素依赖性途径。后期促进复合物/环体（APC/C）在进展中起着至关重要的作用。最初在后期，APC/C需要激活蛋白CDC20以靶向分离酶抑制蛋白和细胞周期蛋白B1进行蛋白酶体依赖性降解，但后来需要Cdh1维持其活性状态直至下一个S期。另一个细胞周期蛋白A/Cdk2介导的Cdh1磷酸化也可能阻止Cdh1与APC的活化组装，从而创造了允许APC靶标积累的环境。它作为黏着连接中的主要粘附受体介导Ca2+依赖性嗜同种的各种相互作用。
Physical form
0.01M磷酸盐缓冲盐水溶液，pH 7.4，含有15 mM叠氮化钠
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
未找到合适的产品？
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Suyang Zhang et al.
Nature, 533(7602), 260-264 (2016-04-28)
In eukaryotes, the anaphase-promoting complex (APC/C, also known as the cyclosome) regulates the ubiquitin-dependent proteolysis of specific cell-cycle proteins to coordinate chromosome segregation in mitosis and entry into the G1 phase. The catalytic activity of the APC/C and its ability
The tumor-suppressor function of E-cadherin.
H Semb et al.
American journal of human genetics, 63(6), 1588-1593 (1998-12-05)
Reinhard Sigl et al.
Journal of cell science, 122(Pt 22), 4208-4217 (2009-10-29)
The anaphase-promoting complex/cyclosome (APC/C) is essential for progression through mitosis. At anaphase onset, the APC/C requires the activator protein CDC20 to target securin and cyclin B1 for proteasome-dependent degradation, but then depends on the CDC20-related protein FZR1 (also known as
C Lukas et al.
Nature, 401(6755), 815-818 (1999-11-05)
In mammalian somatic-cell cycles, progression through the G1-phase restriction point and initiation of DNA replication are controlled by the ability of the retinoblastoma tumour-suppressor protein (pRb) family to regulate the E2F/DP transcription factors. Continuing transcription of E2F target genes beyond
Nozomi Sugimoto et al.
Molecular biology of the cell, 19(3), 1007-1021 (2007-12-29)
In mammalian cells, Cdt1 activity is strictly controlled by multiple independent mechanisms, implying that it is central to the regulation of DNA replication during the cell cycle. In fact, unscheduled Cdt1 hyperfunction results in rereplication and/or chromosomal damage. Thus, it
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持