产品名称
单克隆抗-胶原，X型 小鼠抗, clone COL-10, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
COL-10, monoclonal
mol wt
antigen 60 kDa (in denatured-reduced preparations)
contains
15 mM sodium azide
species reactivity
deer, human, pig
technique(s)
dot blot: suitable
immunocytochemistry: 1:1,000 using HT 1080 human fibrosarcoma cells
western blot: suitable
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL10A1(1300)
Application
小鼠X型抗胶原蛋白单克隆抗体可用于
- 酶联免疫吸附测定（ELISA）
- 斑点印迹
- 免疫印迹和
- 免疫组化。
Biochem/physiol Actions
X型胶原是肥大软骨细胞的产物。X型胶原是非纤维状的，但形成与软骨胶原有关的细的细胞周细丝。它与基质蛋白（如连接蛋白V、软骨钙黄绿素、胶原II和蛋白聚糖）以及Ca2+相互作用。它在软骨内骨化过程中充当避免局部衰竭的支架，并且在软骨矿化中也起作用。人α1（X）胶原基因的NC1编码域中的突变与Schmid干骺端软骨形成有关。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
抗X型胶原蛋白单克隆抗体（小鼠IgM同种型）源自COL-10杂交瘤，该杂交瘤是通过将小鼠骨髓瘤细胞与用X型猪胶原蛋白免疫的BALB/c小鼠的脾细胞融合而产生的。X型胶原蛋白具有相似的结构域结构与VIII型胶原蛋白：50 kDa的中央三螺旋（COL1）结构域两侧是N端（NC2）和C端（NC1）非三螺旋结构域。
胶原蛋白是由三种多肽组成的细胞外糖蛋白，它们混合形成三重螺旋。X型胶原蛋白是在长骨，椎骨和肋骨生长区的胎儿肥大软骨中发现的59 kDα1（X）链的同源三聚体，而在成年人中，它也存在于甲状软骨中。单克隆抗胶原X型抗体可用于细胞培养物的组织学和免疫组织化学评估。它也可用于研究特定的差异组织表达。
Immunogen
纯化的猪X型胶原。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源生物产品
此项目有
Christopher R Rowland et al.
Biomaterials, 91, 57-72 (2016-03-22)
The native extracellular matrix of cartilage contains entrapped growth factors as well as tissue-specific epitopes for cell-matrix interactions, which make it a potentially attractive biomaterial for cartilage tissue engineering. A limitation to this approach is that the native cartilage extracellular
Schmid type of metaphyseal chondrodysplasia and COL10A1 mutations?findings in 10 patients
Makitie O, et al.
American Journal of Medical Genetics. Part A, 137(3), 241-248 (2005)
Aasma A Khan et al.
Biotechnology progress, 25(2), 508-515 (2009-03-19)
The use of bioreactors for cartilage tissue engineering has become increasingly important as traditional batch-fed culture is not optimal for in vitro tissue growth. Most tissue engineering bioreactors rely on convection as the primary means to provide mass transfer; however
Temporal and spatial modulation of chondrogenic foci in subchondral microdrill holes by chitosan-glycerol phosphate/blood implants
Chevrier A, et al.
Osteoarthritis and Cartilage, 19(1), 136-144 (2011)
Mohammad Mardani et al.
Iranian journal of basic medical sciences, 16(11), 1163-1169 (2014-02-05)
Platelet-rich plasma (PRP) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. Little is known about the role of PRP as a promoter in chondrogenesis of human adipose derived stem cells (hADSCs).
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