recombinant
expressed in E. coli
form
liquid
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)
CRISPR
selection
blasticidin
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
Functional Genomics/Screening /Target Validation/Genome Editing
Features and Benefits
Use lentiviral particles to generate cells with stable expression of Cas9 nuclease.
General description
This product is a neomycin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma′s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma′s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link above to order gRNA.
Other Notes
Click here to order gRNA plasmid and virus.
1ug of plasmid DNA, EF1a-Cas9-2A-Neomycin Lenti
存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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