生物来源
fish caudal trunk
包装
tube of 5 μg 00021712-DNA-5UG
pkg of vial of cells 00021712-1VL
生长模式
Adherent
核型
2n=48, stem-line = 48 with scattering from 36-121
形态学
Fibroblast-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
生化/生理作用
Fish, bluegill fry
The cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings of Bluegill fry (Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including lymphocystis virus, viral haemorrhagic septicaemia virus (VHSV), infectious pancreatic necrosis virus (IPNV) and tadpole edema virus (TEV) of frogs. The cell monolayer is confluent after 48-56 hours using a 1:4 split ratio. Catalogue no. at CCLV (depositor) is RIE 193.
制备说明
EMEM(EBSS) (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120 mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442).
Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; cells replicate at 15-33ºC, optimal temperature 23-26ºC; monolayer is stable for at least 10 days, maintenance medium should contain MEM(EBSS) + 740 mg/l NaHCO3 + 1.2g/l
其他说明
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