生物来源
rabbit kidney
包装
tube of 5 μg 00021715-DNA-5UG
pkg of vial of cells 00021715-1VL
生长模式
Adherent
核型
2n=44, stem-line = 56 (=22%)
形态学
Epithelial-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系来源
Rabbit kidney
细胞系描述
RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.
应用
Virus studies
培养基
传代培养常规
Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ºC; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.
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