Huh-7D 12 Cell Line human

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

Human hepatocellular carcinoma

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

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