biological source
Rat x Rat Hybridoma
manufacturer/tradename
ECACC
packaging
tube of 5 μg 01071008-DNA-5UG, pkg of vial of cells 01071008-1VL
ImmunoDon
LOU rat spleen
Myeloma
Y3 Ag 1.2.3
Reactivity
Anti-Abscisic acid, plant growth hormone
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
S-cis, trans-abscisic acid - 4(1) - p-amino-benzoyl hydrazone-KLH conjugate
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat LOU spleen
This hybridoma is a subclone of AFRC MAC 60 (86052303). The antibody is directed against the plant growth substance cis, trans-abscisic acid. The rate of antibody production has been tested at ECACC. It must be noted that this hybridoma produces less than 2 μg/ml of purified antibody.
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2, 37°C. Require mouse macrophage feeder layers upon resuscitation.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.